Rafael Garcia-Mata
-
Associate Professor
Harold C. and Charlotte L. Shaffer Chair in Biological Sciences
Professor, Biological Sciences , College of Natural Sciences and Mathematics
Contact Info
Overview
overview
- I'm a Professor of Biology and a Harold C. and Charlotte L. Shaffer Chair in Biological Sciences. I have trained as a Cell Biologist, first as a PhD students at the University of Alabama at Birmimgham under the supervision of Elizabeth Sztul, and then as a postdoctoral fellow at UNC Chapel Hill under the supervsion of Dr. Keith Burridge. The research in the lab is centered in understanding the mechanisms of regulation of Rho family of small GTPases. Rho GTPases control many aspects of cell behavior, such as the organization of the cytoskeleton, cell migration, cell–cell and cell–matrix adhesion, cell cycle progression, gene expression and cell polarity. Our lab is particularly interested in the role of Rho GTPases during cell adhesion, migration and cell invasion in both normal and cancer cells. I have consistently funded by NIH and other sources throughout the years, and have published over 60 peer reviewed publications that haven been cited more than 7000 times. I have also contributed by doing outreach, including the SCOPE program and Toledo CellulART. I have co-founded the Cell Architecture and Dynamics group, which has been recognized as an area of strength by the University of Toledo. I have also contributed as grant and manuscripot reviewer, for many funding agencies and journals. Finally, I have a strong record of mentoring both graduate and undergraduate students in the lab.
Publications
selected publications
-
Article (Faculty180)
-
2024From pipette to palette: Bridging science and art at MBoC. Molecular Biology of the Cell. 35.
-
2024The Scribble/SGEF/Dlg1 complex regulates the stability of apical junctions in epithelial cells. bioRxiv : the preprint server for biology.
-
2023ARHGAP17 regulates the spatiotemporal activity of Cdc42 at invadopodia. The Journal of cell biology. 222.
-
2023Author Correction: The RhoA dependent anti-metastatic function of RKIP in breast cancer. Scientific reports. 13:11293.
-
2023Cell-based optimization and characterization of genetically encoded location-based biosensors for Cdc42 or Rac activity. Journal of cell science. 136.
-
2023Deciphering Cell-specific Effect of Osteoblast-Macrophage Crosstalk in Periodontitis. Tissue engineering. Part A.
-
2022Nonredundant Rac-GEF control of actin cytoskeleton reorganization. Trends in cell biology. 32:815-818.
-
2022Quantification of ruffle area and dynamics in live or fixed lung adenocarcinoma cells. STAR protocols. 3:101437.
-
2021The RhoA dependent anti-metastatic function of RKIP in breast cancer. Scientific reports. 11:17455.
-
2020Evaluation of active Rac1 levels in cancer cells: A case of misleading conclusions from immunofluorescence analysis. The Journal of biological chemistry. 295:13698-13710.
-
2020Syndecan-4/PAR-3 signaling regulates focal adhesion dynamics in mesenchymal cells. Cell communication and signaling : CCS. 18:129.
-
2019The small GTPase RhoG regulates microtubule-mediated focal adhesion disassembly. Scientific Reports. 9:5163.
-
2017Regulation of circular dorsal ruffles, macropinocytosis and cell migration by RhoG and its exchange factor Trio. Molecular Biology of the Cell. mbc.
-
2017mDia2 and CXCL12/CXCR4 chemokine signaling intersect to drive tumor cell amoeboid morphological transitions. Biochemical and biophysical research communications. 484:255.
-
2014Isolated nuclei adapt to force and reveal a mechanotransduction pathway in the nucleus. Nature Cell Biology. 16:376.
-
2014VAV3 mediates resistance to breast cancer endocrine therapy. Breast Cancer Research. 16:R53.
-
2013Simvastatin inhibits secretion of Th17-polarizing cytokines and antigen presentation by DCs in patients with relapsing remitting multiple sclerosis. European Journal of Immunology. 43:281-289.
-
2012Affinity precipitation of active Rho-GEFs using a GST-tagged mutant Rho protein (GST-RhoA (G17A)) from epithelial cell lysates. Journal of visualized experiments: JoVE. e3932.
-
2012Analysis of the role of RhoGDI1 and isoprenylation in the degradation of RhoGTPases. Rho GTPases: Methods and Protocols. 827:97-105.
-
2012The invasive capacity of HPV transformed cells requires the hDlg-dependent enhancement of SGEF/RhoG activity. PLoS pathogens. 8:e1002543.
-
2011Analysis of RhoA and Rho GEF activity in whole cells and the cell nucleus. Nature Protocols. 6:2050-2060.
-
2011Plexin-B2 negatively regulates macrophage motility, Rac, and Cdc42 activation. PLoS One. 6:e24795.
-
2011
-
2009Palladin contributes to invasive motility in human breast cancer cells. Oncogene. 28:587-598.
-
2007The nuclear RhoA exchange factor Net1 interacts with proteins of the Dlg family, affects their localization, and influences their tumor suppressor activity. Molecular and Cellular Biology. 27:8683.
-
2006Analysis of activated GAPs and GEFs in cell lysates. Methods in enzymology. 406:425.
-
2006Palladin binds to Eps8 and enhances the formation of dorsal ruffles and podosomes in vascular smooth muscle cells. Journal of Cell Science. 119:3316.
-
2005
-
2003ADP-ribosylation factor/COPI-dependent events at the endoplasmic reticulum-Golgi interface are regulated by the guanine nucleotide exchange factor GBF1. Molecular Biology of the Cell. 14:2250.
-
2003Membrane targeting of p115 phosphorylation mutants and their effects on Golgi integrity and secretory traffic. European journal of cell biology. 82:411.
-
2003
-
2002Inhibition of proteasomes induces accumulation, phosphorylation, and recruitment of HSP27 and alphaB-crystallin to aggresomes. The Journal of Biochemistry. 131:593.
-
2001The p115-interactive proteins GM130 and giantin participate in endoplasmic reticulum-Golgi traffic. Journal of Biological Chemistry. 276:2693.
-
2000The membrane transport factor p115 recycles only between homologous compartments in intact heterokaryons. European journal of cell biology. 79:229.
-
1999Characterization and Dynamics of Aggresome Formation by a Cytosolic Gfp-Chimera. The Journal of Cell Biology. 146:1239.
-
1998
-
1997Protein depletion and refeeding change the proportion of mouse liver glutathione S-transferase subunits. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research. 1357:272.
-
-
Article (Web of Science)
-
2023Deciphering the Cell-Specific Effect of Osteoblast-Macrophage Crosstalk in Periodontitis. TISSUE ENGINEERING PART A. 29:579-593.Full Text via DOI: 10.1089/ten.tea.2023.0104 PMID: 37639358
-
2023Cell-based optimization and characterization of genetically encoded location-based biosensors for Cdc42 or Rac activity. JOURNAL OF CELL SCIENCE. 136.Full Text via DOI: 10.1242/jcs.260802 PMID: 37226883
-
2022ARHGAP17 regulates the spatiotemporal activity of Cdc42 at invadopodia. JOURNAL OF CELL BIOLOGY. 222.Full Text via DOI: 10.1083/jcb.202207020 PMID: 36571786
-
2022Mechanoresponsive regulation of fibroblast-to-myofibroblast transition in three-dimensional tissue analogues: mechanical strain amplitude dependency of fibrosis. SCIENTIFIC REPORTS. 12.Full Text via DOI: 10.1038/s41598-022-20383-5 PMID: 36207437
-
2022Fixing the GAP: The role of RhoGAPs in cancer. EUROPEAN JOURNAL OF CELL BIOLOGY. 101.Full Text via DOI: 10.1016/j.ejcb.2022.151209 PMID: 35180567
-
2022Quantification of ruffle area and dynamics in live or fixed lung adenocarcinoma cells. STAR PROTOCOLS. 3:101437.Full Text via DOI: 10.1016/j.xpro.2022.101437
-
2021FARP1, ARHGEF39, and TIAM2 are essential receptor tyrosine kinase effectors for Rac1-dependent cell motility in human lung adenocarcinoma. CELL REPORTS. 37.Full Text via DOI: 10.1016/j.celrep.2021.109905 PMID: 34731623
-
2021The RhoA dependent anti-metastatic function of RKIP in breast cancer. SCIENTIFIC REPORTS. 11.Full Text via DOI: 10.1038/s41598-021-96709-6 PMID: 34465801
-
2021ARHGEF26 enhances Salmonella invasion and inflammation in cells and mice. PLOS PATHOGENS. 17.Full Text via DOI: 10.1371/journal.ppat.1009713 PMID: 34242364
-
2020Syndecan-4/PAR-3 signaling regulates focal adhesion dynamics in mesenchymal cells. CELL COMMUNICATION AND SIGNALING. 18.Full Text via DOI: 10.1186/s12964-020-00629-3 PMID: 32811537
-
2020P-REX1-Independent, Calcium-Dependent RAC1 Hyperactivation in Prostate Cancer. CANCERS. 12.Full Text via DOI: 10.3390/cancers12020480 PMID: 32092966
-
2020Evaluation of active Rac1 levels in cancer cells: A case of misleading conclusions from immunofluorescence analysis. JOURNAL OF BIOLOGICAL CHEMISTRY. 295:13698-13710.Full Text via DOI: 10.1074/jbc.ra120.013919
-
2019SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility. JOURNAL OF CELL BIOLOGY. 218:2699-2725.Full Text via DOI: 10.1083/jcb.201811114 PMID: 31248911
-
2019The small GTPase RhoG regulates microtubule-mediated focal adhesion disassembly. SCIENTIFIC REPORTS. 9.Full Text via DOI: 10.1038/s41598-019-41558-7 PMID: 30914742
-
2017Regulation of circular dorsal ruffles, macropinocytosis, and cell migration by RhoG and its exchange factor, Trio. MOLECULAR BIOLOGY OF THE CELL. 28:1768-1781.Full Text via DOI: 10.1091/mbc.E16-06-0412
-
2017A RhoG-mediated signaling pathway that modulates invadopodia dynamics in breast cancer cells. JOURNAL OF CELL SCIENCE. 130:1064-1077.Full Text via DOI: 10.1242/jcs.195552
-
2017mDia2 and CXCL12/CXCR4 chemokine signaling intersect to drive tumor cell amoeboid morphological transitions. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 484:255-261.Full Text via DOI: 10.1016/j.bbrc.2017.01.087
-
2017RNase L Suppresses Androgen Receptor Signaling, Cell Migration and Matrix Metalloproteinase Activity in Prostate Cancer Cells. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 18.Full Text via DOI: 10.3390/ijms18030529
-
2017Regulation of circular dorsal ruffles, macropinocytosis, and cell migration by RhoG and its exchange factor, Trio. MOLECULAR BIOLOGY OF THE CELL. 28:1768-1781.Full Text via DOI: 10.1091/mbc.e16-06-0412
-
2014Isolated nuclei adapt to force and reveal a mechanotransduction pathway in the nucleus. NATURE CELL BIOLOGY. 16:376-381.Full Text via DOI: 10.1038/ncb2927
-
2014Palladin promotes invasion of pancreatic cancer cells by enhancing invadopodia formation in cancer-associated fibroblasts. ONCOGENE. 33:1265-1273.Full Text via DOI: 10.1038/onc.2013.68
-
2014VAV3 mediates resistance to breast cancer endocrine therapy. Breast Cancer Research. 16:R53.Full Text via DOI: 10.1186/bcr3664
-
2013Simvastatin inhibits secretion of Th17-polarizing cytokines and antigen presentation by DCs in patients with relapsing remitting multiple sclerosis. EUROPEAN JOURNAL OF IMMUNOLOGY. 43:281-289.Full Text via DOI: 10.1002/eji.201242566
-
2012Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS.Full Text via DOI: 10.3791/3932
-
2012The Invasive Capacity of HPV Transformed Cells Requires the hDlg-Dependent Enhancement of SGEF/RhoG Activity. PLOS Pathogens. 8:e1002543.Full Text via DOI: 10.1371/journal.ppat.1002543
-
2012The UDP-sugar-sensing P2Y14receptor promotes Rho-mediated signaling and chemotaxis in human neutrophils. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY. 303:C490-C498.Full Text via DOI: 10.1152/ajpcell.00138.2012
-
2011Analysis of RhoA and Rho GEF activity in whole cells and the cell nucleus. Nature Protocols. 6:2050-2060.Full Text via DOI: 10.1038/nprot.2011.411
-
2011Plexin-B2 Negatively Regulates Macrophage Motility, Rac, and Cdc42 Activation. PLOS ONE. 6:e24795.Full Text via DOI: 10.1371/journal.pone.0024795
-
2011The Rho GEFs LARG and GEF-H1 regulate the mechanical response to force on integrins. NATURE CELL BIOLOGY. 13:722-727.Full Text via DOI: 10.1038/ncb2254
-
2011The Small GTPase RhoA Localizes to the Nucleus and Is Activated by Net1 and DNA Damage Signals. PLOS ONE. 6:e17380.Full Text via DOI: 10.1371/journal.pone.0017380
-
2010Regulation of Rho GTPase crosstalk, degradation and activity by RhoGDI1. NATURE CELL BIOLOGY. 12:477-483.Full Text via DOI: 10.1038/ncb2049
-
2009Palladin contributes to invasive motility in human breast cancer cells. ONCOGENE. 28:587-598.Full Text via DOI: 10.1038/onc.2008.408
-
2007A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA activity downstream of adhesion to fibronectin. JOURNAL OF CELL SCIENCE. 120:3989-3998.Full Text via DOI: 10.1242/jcs.003806
-
2007RhoG regulates endothelial apical cup assembly downstream from ICAM1 engagement and is involved in leukocyte trans-endothelial migration. JOURNAL OF CELL BIOLOGY. 178:1279-1293.Full Text via DOI: 10.1083/jcb.200612053
-
2007The Nuclear RhoA Exchange Factor Net1 Interacts with Proteins of the Dlg Family, Affects Their Localization, and Influences Their Tumor Suppressor Activity. MOLECULAR AND CELLULAR BIOLOGY. 27:8683-8697.Full Text via DOI: 10.1128/mcb.00157-07
-
2006Palladin binds to Eps8 and enhances the formation of dorsal ruffles and podosomes in vascular smooth muscle cells. JOURNAL OF CELL SCIENCE. 119:3316-3324.Full Text via DOI: 10.1242/jcs.03076
-
2005Dissection of Membrane Dynamics of the ARF-Guanine Nucleotide Exchange Factor GBF1. Traffic. 6:374-385.Full Text via DOI: 10.1111/j.1600-0854.2005.00282.x
-
2003ADP-Ribosylation Factor/COPI-dependent Events at the Endoplasmic Reticulum-Golgi Interface Are Regulated by the Guanine Nucleotide Exchange Factor GBF1. MOLECULAR BIOLOGY OF THE CELL. 14:2250-2261.Full Text via DOI: 10.1091/mbc.e02-11-0730
-
2003COPI Recruitment Is Modulated by a Rab1b-dependent Mechanism. MOLECULAR BIOLOGY OF THE CELL. 14:2116-2127.Full Text via DOI: 10.1091/mbc.e02-09-0625
-
2003Membrane targeting of p115 phosphorylation mutants and their effects on Golgi integrity and secretory traffic. European Journal of Cell Biology. 82:411-420.Full Text via DOI: 10.1078/0171-9335-00327
-
2003The membrane‐tethering protein p115 interacts with GBF1, an ARF guanine‐nucleotide‐exchange factor. EMBO reports. 4:320-325.Full Text via DOI: 10.1038/sj.embor.embor762
-
2002Inhibition of Proteasomes Induces Accumulation, Phosphorylation, and Recruitment of HSP27 and -Crystallin to Aggresomes. JOURNAL OF BIOCHEMISTRY. 131:593-603.Full Text via DOI: 10.1093/oxfordjournals.jbchem.a003139
-
2001The p115-interactive Proteins GM130 and Giantin Participate in Endoplasmic Reticulum-Golgi Traffic. JOURNAL OF BIOLOGICAL CHEMISTRY. 276:2693-2700.Full Text via DOI: 10.1074/jbc.m007957200
-
2000Binding Relationships of Membrane Tethering Components. JOURNAL OF BIOLOGICAL CHEMISTRY. 275:10196-10201.Full Text via DOI: 10.1074/jbc.275.14.10196
-
2000The membrane transport factor p115 recycles only between homologous compartments in intact heterokaryons. European Journal of Cell Biology. 79:229-239.Full Text via DOI: 10.1078/s0171-9335(04)70026-6
-
1999Characterization and Dynamics of Aggresome Formation by a Cytosolic Gfp-Chimera✪. JOURNAL OF CELL BIOLOGY. 146:1239-1254.Full Text via DOI: 10.1083/jcb.146.6.1239
-
1998Contribution of breakdown in the regulation of mouse liver glutathione S-transferase subunits during protein depletion and re-feeding. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH. 1448:46-50.Full Text via DOI: 10.1016/s0167-4889(98)00117-7
-
1998The Membrane Transport Factor TAP/p115 Cycles between the Golgi and Earlier Secretory Compartments and Contains Distinct Domains Required for Its Localization and Function. JOURNAL OF CELL BIOLOGY. 143:319-331.Full Text via DOI: 10.1083/jcb.143.2.319
-
1997Protein depletion and refeeding change the proportion of mouse liver glutathione S-transferase subunits. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH. 1357:272-280.Full Text via DOI: 10.1016/s0167-4889(97)00036-0
-
-
Book Chapter (Web of Science)
-
2012Analysis of the Role of RhoGDI1 and Isoprenylation in the Degradation of RhoGTPases. Methods in Molecular Biology. 97-105.Full Text via DOI: 10.1007/978-1-61779-442-1_7
-
2009Chapter 1 Focal Adhesions: New Angles on an Old Structure. International Review of Cell and Molecular Biology. 1-65.Full Text via DOI: 10.1016/s1937-6448(09)77001-7
-
2006Analysis of Activated GAPs and GEFs in Cell Lysates. Methods in Enzymology. 425-437.Full Text via DOI: 10.1016/s0076-6879(06)06031-9
-
-
Conference Poster (Faculty180)
-
2019
-
Editorial Material (Web of Science)
-
2014Arrested Detachment: A DEPDC1B-Mediated De-adhesion Mitotic Checkpoint. DEVELOPMENTAL CELL. 387-389.Full Text via DOI: 10.1016/j.devcel.2014.11.008
-
-
Meeting Abstract (Web of Science)
-
2018Rational Design of PDZ Domain Specificity. BIOPHYSICAL JOURNAL. 410A-410A.
-
2017Regulation of epithelial junctions and polarity by the Scribble/SGEF/Dlg complex.. MOLECULAR BIOLOGY OF THE CELL.
-
2017RhoG regulates migration through focal adhesion dynamics and contractility.. MOLECULAR BIOLOGY OF THE CELL.
-
2016A RhoG-mediated signaling pathway that modulates invadopodia formation in breast cancer cells.. MOLECULAR BIOLOGY OF THE CELL.
-
2016Regulation of circular dorsal ruffles, macropinocytosis and cell migration by RhoG and its exchange factor Trio.. MOLECULAR BIOLOGY OF THE CELL.
-
2016The RhoG exchange factor SGEF forms a ternary complex with two members of the SCRIBBLE polarity complex, Scribble and Dlg1.. MOLECULAR BIOLOGY OF THE CELL.
-
2016The small GTPase RhoG modulates focal adhesion turnover.. MOLECULAR BIOLOGY OF THE CELL.
-
2014RhoG Negatively Regulates Invadopodia Formation.. MOLECULAR BIOLOGY OF THE CELL.
-
2013CXCL12/CXCR4 drives migratory plasticity through a RhoA-directed mDia2/DIP signaling hub in human breast cancer cells. MOLECULAR BIOLOGY OF THE CELL.
-
2013Emerin regulates the mechanical response to force on nesprin-1 in isolated nuclei. MOLECULAR BIOLOGY OF THE CELL.
-
2012A novel PDGF-mediated RhoG pathway in vascular smooth muscle cells.. MOLECULAR BIOLOGY OF THE CELL.
-
2012Palladin Cycles Between the Stress Fibers and the Nucleus to Regulate Transcription During Myofibroblast Differentiation. MOLECULAR BIOLOGY OF THE CELL.
-
2012Palladin expression modulates RhoG activation, migration and invasion of breast cancer cells.. MOLECULAR BIOLOGY OF THE CELL.
-
-
Presentation (Faculty180)
-
Review (Faculty180)
-
2012
-
2011
-
2010
-
2007
-
Review Article (Web of Science)
-
2014I'm coming to GEF you Regulation of RhoGEFs during cell migration. CELL ADHESION & MIGRATION. 535-549.Full Text via DOI: 10.4161/cam.28721
-
2012Golgi Membrane Dynamics and Lipid Metabolism. CURRENT BIOLOGY. R414-R424.Full Text via DOI: 10.1016/j.cub.2012.03.004
-
2012Off the beaten paths: alternative and crosstalk regulation of Rho GTPases. FASEB JOURNAL. 469-479.Full Text via DOI: 10.1096/fj.11-192252
-
2011Rho protein crosstalk: another social network?. Trends in Cell Biology. 718-726.Full Text via DOI: 10.1016/j.tcb.2011.08.002
-
2011The 'invisible hand': regulation of RHO GTPases by RHOGDIs. Nature Reviews Molecular Cell Biology. 493-504.Full Text via DOI: 10.1038/nrm3153
-
2007Catching a GEF by its tail. Trends in Cell Biology. 36-43.Full Text via DOI: 10.1016/j.tcb.2006.11.004
-
2002Hassles with Taking Out the Garbage: Aggravating Aggresomes. Traffic. 388-396.Full Text via DOI: 10.1034/j.1600-0854.2002.30602.x
-
Contact
full name
- Rafael Garcia-Mata
visualizations
Cumulative publications in Scholars@UToledo