Rafael Garcia-Mata
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Associate Professor
Harold C. and Charlotte L. Shaffer Chair in Biological Sciences
Professor, Biological Sciences , College of Natural Sciences and Mathematics
Contact Info
Publications
selected publications
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Article (Faculty180)
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2023ARHGAP17 regulates the spatiotemporal activity of Cdc42 at invadopodia. The Journal of cell biology. 222.
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2023Author Correction: The RhoA dependent anti-metastatic function of RKIP in breast cancer. Scientific reports. 13:11293.
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2023Cell-based optimization and characterization of genetically encoded location-based biosensors for Cdc42 or Rac activity. Journal of cell science. 136.
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2023Macrophage Mechanoresponsiveness within 3D Tissue Matrix upon Mechanotherapy- Associated Strains. Tissue engineering. Part A.
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2022Fixing the GAP: The role of RhoGAPs in cancer. European journal of cell biology. 101:151209.
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2022Nonredundant Rac-GEF control of actin cytoskeleton reorganization. Trends in cell biology. 32:815-818.
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2022Quantification of ruffle area and dynamics in live or fixed lung adenocarcinoma cells. STAR protocols. 3:101437.
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2021ARHGEF26 enhances Salmonella invasion and inflammation in cells and mice. PLoS pathogens. 17:e1009713.
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2021The RhoA dependent anti-metastatic function of RKIP in breast cancer. Scientific reports. 11:17455.
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2020Evaluation of active Rac1 levels in cancer cells: A case of misleading conclusions from immunofluorescence analysis. The Journal of biological chemistry. 295:13698-13710.
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2020Syndecan-4/PAR-3 signaling regulates focal adhesion dynamics in mesenchymal cells. Cell communication and signaling : CCS. 18:129.
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2019SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility. The Journal of Cell Biology. 218:2699-2725.
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2019The small GTPase RhoG regulates microtubule-mediated focal adhesion disassembly. Scientific Reports. 9:5163.
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2017A RhoG-mediated signaling pathway that modulates invadopodia dynamics in breast cancer cells. Journal of Cell Science. 130:1064-1077.
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2017RNase L Suppresses Androgen Receptor Signaling, Cell Migration and Matrix Metalloproteinase Activity in Prostate Cancer Cells. International journal of molecular sciences. 18:529.
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2017Regulation of circular dorsal ruffles, macropinocytosis and cell migration by RhoG and its exchange factor Trio. Molecular Biology of the Cell. mbc.
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2017mDia2 and CXCL12/CXCR4 chemokine signaling intersect to drive tumor cell amoeboid morphological transitions. Biochemical and biophysical research communications. 484:255.
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2014Isolated nuclei adapt to force and reveal a mechanotransduction pathway in the nucleus. Nature Cell Biology. 16:376.
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2014VAV3 mediates resistance to breast cancer endocrine therapy. Breast Cancer Research. 16:R53.
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2013Simvastatin inhibits secretion of Th17-polarizing cytokines and antigen presentation by DCs in patients with relapsing remitting multiple sclerosis. European Journal of Immunology. 43:281-289.
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2012Affinity precipitation of active Rho-GEFs using a GST-tagged mutant Rho protein (GST-RhoA (G17A)) from epithelial cell lysates. Journal of visualized experiments: JoVE. e3932.
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2012Analysis of the role of RhoGDI1 and isoprenylation in the degradation of RhoGTPases. Rho GTPases: Methods and Protocols. 827:97-105.
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2012The UDP-sugar-sensing P2Y 14 receptor promotes Rho-mediated signaling and chemotaxis in human neutrophils. American Journal of Physiology-Cell Physiology. 303:C490.
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2012The invasive capacity of HPV transformed cells requires the hDlg-dependent enhancement of SGEF/RhoG activity. PLoS pathogens. 8:e1002543.
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2011Analysis of RhoA and Rho GEF activity in whole cells and the cell nucleus. Nature Protocols. 6:2050-2060.
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2011Plexin-B2 negatively regulates macrophage motility, Rac, and Cdc42 activation. PLoS One. 6:e24795.
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2011The Rho GEFs LARG and GEF-H1 regulate the mechanical response to force on integrins. Nature Cell Biology. 13:722.
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2011
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2010Regulation of Rho GTPase crosstalk, degradation and activity by RhoGDI1. Nature Cell Biology. 12:477-483.
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2009Palladin contributes to invasive motility in human breast cancer cells. Oncogene. 28:587-598.
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2007A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA activity downstream of adhesion to fibronectin. Journal of Cell Science. 120:3989.
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2007The nuclear RhoA exchange factor Net1 interacts with proteins of the Dlg family, affects their localization, and influences their tumor suppressor activity. Molecular and Cellular Biology. 27:8683.
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2006Analysis of activated GAPs and GEFs in cell lysates. Methods in enzymology. 406:425.
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2006Palladin binds to Eps8 and enhances the formation of dorsal ruffles and podosomes in vascular smooth muscle cells. Journal of Cell Science. 119:3316.
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2005
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2003ADP-ribosylation factor/COPI-dependent events at the endoplasmic reticulum-Golgi interface are regulated by the guanine nucleotide exchange factor GBF1. Molecular Biology of the Cell. 14:2250.
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2003COPI recruitment is modulated by a Rab1b-dependent mechanism. Molecular Biology of the Cell. 14:2116.
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2003Membrane targeting of p115 phosphorylation mutants and their effects on Golgi integrity and secretory traffic. European journal of cell biology. 82:411.
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2003
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2002Inhibition of proteasomes induces accumulation, phosphorylation, and recruitment of HSP27 and alphaB-crystallin to aggresomes. The Journal of Biochemistry. 131:593.
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2001The p115-interactive proteins GM130 and giantin participate in endoplasmic reticulum-Golgi traffic. Journal of Biological Chemistry. 276:2693.
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2000Binding relationships of membrane tethering components The giantin N terminus and the GM130 N terminus compete for binding to the p115 C terminus. Journal of Biological Chemistry. 275:10196.
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2000The membrane transport factor p115 recycles only between homologous compartments in intact heterokaryons. European journal of cell biology. 79:229.
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1999Characterization and Dynamics of Aggresome Formation by a Cytosolic Gfp-Chimera. The Journal of Cell Biology. 146:1239.
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1998Contribution of breakdown in the regulation of mouse liver glutathione S-transferase subunits during protein depletion and re-feeding. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research. 1448:46.
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1998
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1997Protein depletion and refeeding change the proportion of mouse liver glutathione S-transferase subunits. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research. 1357:272.
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Article (Web of Science)
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2021FARP1, ARHGEF39, and TIAM2 are essential receptor tyrosine kinase effectors for Rac1-dependent cell motility in human lung adenocarcinoma. CELL REPORTS. 37.Full Text via DOI: 10.1016/j.celrep.2021.109905 PMID: 34731623
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2021The RhoA dependent anti-metastatic function of RKIP in breast cancer. SCIENTIFIC REPORTS. 11.Full Text via DOI: 10.1038/s41598-021-96709-6 PMID: 34465801
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2021ARHGEF26 enhances Salmonella invasion and inflammation in cells and mice. PLOS PATHOGENS. 17.Full Text via DOI: 10.1371/journal.ppat.1009713 PMID: 34242364
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2020Syndecan-4/PAR-3 signaling regulates focal adhesion dynamics in mesenchymal cells. CELL COMMUNICATION AND SIGNALING. 18.Full Text via DOI: 10.1186/s12964-020-00629-3 PMID: 32811537
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2020P-REX1-Independent, Calcium-Dependent RAC1 Hyperactivation in Prostate Cancer. CANCERS. 12.Full Text via DOI: 10.3390/cancers12020480 PMID: 32092966
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2020Evaluation of active Rac1 levels in cancer cells: A case of misleading conclusions from immunofluorescence analysis. JOURNAL OF BIOLOGICAL CHEMISTRY. 295:13698-13710.Full Text via DOI: 10.1074/jbc.ra120.013919
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2019SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility. JOURNAL OF CELL BIOLOGY. 218:2699-2725.Full Text via DOI: 10.1083/jcb.201811114 PMID: 31248911
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2019The small GTPase RhoG regulates microtubule-mediated focal adhesion disassembly. SCIENTIFIC REPORTS. 9.Full Text via DOI: 10.1038/s41598-019-41558-7 PMID: 30914742
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2017Regulation of circular dorsal ruffles, macropinocytosis, and cell migration by RhoG and its exchange factor, Trio. MOLECULAR BIOLOGY OF THE CELL. 28:1768-1781.Full Text via DOI: 10.1091/mbc.E16-06-0412
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2017A RhoG-mediated signaling pathway that modulates invadopodia dynamics in breast cancer cells. JOURNAL OF CELL SCIENCE. 130:1064-1077.Full Text via DOI: 10.1242/jcs.195552
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2017mDia2 and CXCL12/CXCR4 chemokine signaling intersect to drive tumor cell amoeboid morphological transitions. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 484:255-261.Full Text via DOI: 10.1016/j.bbrc.2017.01.087
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2017RNase L Suppresses Androgen Receptor Signaling, Cell Migration and Matrix Metalloproteinase Activity in Prostate Cancer Cells. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 18.Full Text via DOI: 10.3390/ijms18030529
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2017Regulation of circular dorsal ruffles, macropinocytosis, and cell migration by RhoG and its exchange factor, Trio. MOLECULAR BIOLOGY OF THE CELL. 28:1768-1781.Full Text via DOI: 10.1091/mbc.e16-06-0412
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2014Isolated nuclei adapt to force and reveal a mechanotransduction pathway in the nucleus. NATURE CELL BIOLOGY. 16:376-381.Full Text via DOI: 10.1038/ncb2927
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2014Palladin promotes invasion of pancreatic cancer cells by enhancing invadopodia formation in cancer-associated fibroblasts. ONCOGENE. 33:1265-1273.Full Text via DOI: 10.1038/onc.2013.68
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2014VAV3 mediates resistance to breast cancer endocrine therapy. Breast Cancer Research. 16:R53.Full Text via DOI: 10.1186/bcr3664
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2013Simvastatin inhibits secretion of Th17-polarizing cytokines and antigen presentation by DCs in patients with relapsing remitting multiple sclerosis. EUROPEAN JOURNAL OF IMMUNOLOGY. 43:281-289.Full Text via DOI: 10.1002/eji.201242566
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2012Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS.Full Text via DOI: 10.3791/3932
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2012The Invasive Capacity of HPV Transformed Cells Requires the hDlg-Dependent Enhancement of SGEF/RhoG Activity. PLOS Pathogens. 8:e1002543.Full Text via DOI: 10.1371/journal.ppat.1002543
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2012The UDP-sugar-sensing P2Y14receptor promotes Rho-mediated signaling and chemotaxis in human neutrophils. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY. 303:C490-C498.Full Text via DOI: 10.1152/ajpcell.00138.2012
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2011Analysis of RhoA and Rho GEF activity in whole cells and the cell nucleus. Nature Protocols. 6:2050-2060.Full Text via DOI: 10.1038/nprot.2011.411
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2011Plexin-B2 Negatively Regulates Macrophage Motility, Rac, and Cdc42 Activation. PLOS ONE. 6:e24795.Full Text via DOI: 10.1371/journal.pone.0024795
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2011The Rho GEFs LARG and GEF-H1 regulate the mechanical response to force on integrins. NATURE CELL BIOLOGY. 13:722-727.Full Text via DOI: 10.1038/ncb2254
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2011The Small GTPase RhoA Localizes to the Nucleus and Is Activated by Net1 and DNA Damage Signals. PLOS ONE. 6:e17380.Full Text via DOI: 10.1371/journal.pone.0017380
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2010Regulation of Rho GTPase crosstalk, degradation and activity by RhoGDI1. NATURE CELL BIOLOGY. 12:477-483.Full Text via DOI: 10.1038/ncb2049
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2009Palladin contributes to invasive motility in human breast cancer cells. ONCOGENE. 28:587-598.Full Text via DOI: 10.1038/onc.2008.408
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2007A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA activity downstream of adhesion to fibronectin. JOURNAL OF CELL SCIENCE. 120:3989-3998.Full Text via DOI: 10.1242/jcs.003806
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2007RhoG regulates endothelial apical cup assembly downstream from ICAM1 engagement and is involved in leukocyte trans-endothelial migration. JOURNAL OF CELL BIOLOGY. 178:1279-1293.Full Text via DOI: 10.1083/jcb.200612053
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2007The Nuclear RhoA Exchange Factor Net1 Interacts with Proteins of the Dlg Family, Affects Their Localization, and Influences Their Tumor Suppressor Activity. MOLECULAR AND CELLULAR BIOLOGY. 27:8683-8697.Full Text via DOI: 10.1128/mcb.00157-07
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2006Palladin binds to Eps8 and enhances the formation of dorsal ruffles and podosomes in vascular smooth muscle cells. JOURNAL OF CELL SCIENCE. 119:3316-3324.Full Text via DOI: 10.1242/jcs.03076
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2005Dissection of Membrane Dynamics of the ARF-Guanine Nucleotide Exchange Factor GBF1. Traffic. 6:374-385.Full Text via DOI: 10.1111/j.1600-0854.2005.00282.x
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2003ADP-Ribosylation Factor/COPI-dependent Events at the Endoplasmic Reticulum-Golgi Interface Are Regulated by the Guanine Nucleotide Exchange Factor GBF1. MOLECULAR BIOLOGY OF THE CELL. 14:2250-2261.Full Text via DOI: 10.1091/mbc.e02-11-0730
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2003COPI Recruitment Is Modulated by a Rab1b-dependent Mechanism. MOLECULAR BIOLOGY OF THE CELL. 14:2116-2127.Full Text via DOI: 10.1091/mbc.e02-09-0625
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2003Membrane targeting of p115 phosphorylation mutants and their effects on Golgi integrity and secretory traffic. European Journal of Cell Biology. 82:411-420.Full Text via DOI: 10.1078/0171-9335-00327
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2003The membrane‐tethering protein p115 interacts with GBF1, an ARF guanine‐nucleotide‐exchange factor. EMBO reports. 4:320-325.Full Text via DOI: 10.1038/sj.embor.embor762
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2002Inhibition of Proteasomes Induces Accumulation, Phosphorylation, and Recruitment of HSP27 and -Crystallin to Aggresomes. JOURNAL OF BIOCHEMISTRY. 131:593-603.Full Text via DOI: 10.1093/oxfordjournals.jbchem.a003139
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2001The p115-interactive Proteins GM130 and Giantin Participate in Endoplasmic Reticulum-Golgi Traffic. JOURNAL OF BIOLOGICAL CHEMISTRY. 276:2693-2700.Full Text via DOI: 10.1074/jbc.m007957200
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2000Binding Relationships of Membrane Tethering Components. JOURNAL OF BIOLOGICAL CHEMISTRY. 275:10196-10201.Full Text via DOI: 10.1074/jbc.275.14.10196
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2000The membrane transport factor p115 recycles only between homologous compartments in intact heterokaryons. European Journal of Cell Biology. 79:229-239.Full Text via DOI: 10.1078/s0171-9335(04)70026-6
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1999Characterization and Dynamics of Aggresome Formation by a Cytosolic Gfp-Chimera✪. JOURNAL OF CELL BIOLOGY. 146:1239-1254.Full Text via DOI: 10.1083/jcb.146.6.1239
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1998Contribution of breakdown in the regulation of mouse liver glutathione S-transferase subunits during protein depletion and re-feeding. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH. 1448:46-50.Full Text via DOI: 10.1016/s0167-4889(98)00117-7
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1998The Membrane Transport Factor TAP/p115 Cycles between the Golgi and Earlier Secretory Compartments and Contains Distinct Domains Required for Its Localization and Function. JOURNAL OF CELL BIOLOGY. 143:319-331.Full Text via DOI: 10.1083/jcb.143.2.319
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1997Protein depletion and refeeding change the proportion of mouse liver glutathione S-transferase subunits. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH. 1357:272-280.Full Text via DOI: 10.1016/s0167-4889(97)00036-0
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Book Chapter (Web of Science)
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2012Analysis of the Role of RhoGDI1 and Isoprenylation in the Degradation of RhoGTPases. Methods in Molecular Biology. 97-105.Full Text via DOI: 10.1007/978-1-61779-442-1_7
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2009Chapter 1 Focal Adhesions: New Angles on an Old Structure. International Review of Cell and Molecular Biology. 1-65.Full Text via DOI: 10.1016/s1937-6448(09)77001-7
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2006Analysis of Activated GAPs and GEFs in Cell Lysates. Methods in Enzymology. 425-437.Full Text via DOI: 10.1016/s0076-6879(06)06031-9
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Conference Poster (Faculty180)
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2019
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Editorial Material (Web of Science)
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2014Arrested Detachment: A DEPDC1B-Mediated De-adhesion Mitotic Checkpoint. DEVELOPMENTAL CELL. 387-389.Full Text via DOI: 10.1016/j.devcel.2014.11.008
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Meeting Abstract (Web of Science)
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2018Rational Design of PDZ Domain Specificity. BIOPHYSICAL JOURNAL. 410A-410A.
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2017Regulation of epithelial junctions and polarity by the Scribble/SGEF/Dlg complex.. MOLECULAR BIOLOGY OF THE CELL.
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2017RhoG regulates migration through focal adhesion dynamics and contractility.. MOLECULAR BIOLOGY OF THE CELL.
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2016A RhoG-mediated signaling pathway that modulates invadopodia formation in breast cancer cells.. MOLECULAR BIOLOGY OF THE CELL.
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2016Regulation of circular dorsal ruffles, macropinocytosis and cell migration by RhoG and its exchange factor Trio.. MOLECULAR BIOLOGY OF THE CELL.
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2016The RhoG exchange factor SGEF forms a ternary complex with two members of the SCRIBBLE polarity complex, Scribble and Dlg1.. MOLECULAR BIOLOGY OF THE CELL.
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2016The small GTPase RhoG modulates focal adhesion turnover.. MOLECULAR BIOLOGY OF THE CELL.
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2014RhoG Negatively Regulates Invadopodia Formation.. MOLECULAR BIOLOGY OF THE CELL.
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2013CXCL12/CXCR4 drives migratory plasticity through a RhoA-directed mDia2/DIP signaling hub in human breast cancer cells. MOLECULAR BIOLOGY OF THE CELL.
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2013Emerin regulates the mechanical response to force on nesprin-1 in isolated nuclei. MOLECULAR BIOLOGY OF THE CELL.
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2012A novel PDGF-mediated RhoG pathway in vascular smooth muscle cells.. MOLECULAR BIOLOGY OF THE CELL.
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2012Palladin Cycles Between the Stress Fibers and the Nucleus to Regulate Transcription During Myofibroblast Differentiation. MOLECULAR BIOLOGY OF THE CELL.
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2012Palladin expression modulates RhoG activation, migration and invasion of breast cancer cells.. MOLECULAR BIOLOGY OF THE CELL.
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Presentation (Faculty180)
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2013
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Review (Faculty180)
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2012
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2011
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2011
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2010
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2009
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2007
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Review Article (Web of Science)
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2014I'm coming to GEF you Regulation of RhoGEFs during cell migration. CELL ADHESION & MIGRATION. 535-549.Full Text via DOI: 10.4161/cam.28721
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2012Golgi Membrane Dynamics and Lipid Metabolism. CURRENT BIOLOGY. R414-R424.Full Text via DOI: 10.1016/j.cub.2012.03.004
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2012Off the beaten paths: alternative and crosstalk regulation of Rho GTPases. FASEB JOURNAL. 469-479.Full Text via DOI: 10.1096/fj.11-192252
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2011Rho protein crosstalk: another social network?. Trends in Cell Biology. 718-726.Full Text via DOI: 10.1016/j.tcb.2011.08.002
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2011The 'invisible hand': regulation of RHO GTPases by RHOGDIs. Nature Reviews Molecular Cell Biology. 493-504.Full Text via DOI: 10.1038/nrm3153
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2007Catching a GEF by its tail. Trends in Cell Biology. 36-43.Full Text via DOI: 10.1016/j.tcb.2006.11.004
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2002Hassles with Taking Out the Garbage: Aggravating Aggresomes. Traffic. 388-396.Full Text via DOI: 10.1034/j.1600-0854.2002.30602.x
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Contact
full name
- Rafael Garcia-Mata
visualizations
Cumulative publications in Scholars@UToledo
