Striated muscle-specific serine/threonine-protein kinase beta segregates with high versus low responsiveness to endurance exercise training Article (Faculty180)

cited authors

  • Kusić, Denis; Connolly, Joanne; Kainulainen, Heikki; Semenova, Ek A; Borisov, Oleg V; Larin, Andrey K; Popov, Daniil V; Generozov, Edward V; Ahmetov, Ildus I I; Britton, Steven L; Koch, Lauren G; Burniston, Jatin G

description

  • Bidirectional selection for either high or low responsiveness to endurance running has created divergent rat phenotypes of high-response trainers (HRT) and low-response trainers (LRT). We conducted proteome profiling of HRT and LRT gastrocnemius of 10 female rats (body weight 279 ± 35 g; = 5 LRT and = 5 HRT) from of selection. Differential analysis of soluble proteins from gastrocnemius was conducted by label-free quantitation. Genetic association studies were conducted in 384 Russian international-level athletes (age 23.8 ± 3.4 yr; 202 men and 182 women) stratified to endurance or power disciplines. Proteomic analysis encompassed 1,024 proteins, 76 of which exhibited statistically significant ( < 0.05, false discovery rate <1%) differences between HRT and LRT muscle. There was significant enrichment of enzymes involved in glycolysis/gluconeogenesis in LRT muscle but no enrichment of gene ontology phrases in HRT muscle. Striated muscle-specific serine/threonine-protein kinase-beta (SPEG-β) exhibited the greatest difference in abundance and was 2.64-fold greater ( = 0.0014) in HRT muscle. Coimmunoprecipitation identified 24 potential binding partners of SPEG-β in HRT muscle. The frequency of the G variant of the rs7564856 polymorphism that increases gene expression was significantly greater (32.9 vs. 23.8%; OR = 1.6, = 0.009) in international-level endurance athletes ( = 258) compared with power athletes ( = 126) and was significantly associated (β = 8.345, = 0.0048) with a greater proportion of slow-twitch fibers in vastus lateralis of female endurance athletes. Coimmunoprecipitation of SPEG-β in HRT muscle discovered putative interacting proteins that link with previously reported differences in transforming growth factor-β signaling in exercised muscle.

authors

publication date

  • 2020

published in

start page

  • 35

end page

  • 46

volume

  • 52