Peripheral oxygen transport and utilization in rats following continued selective breeding for endurance running capacity Article (Web of Science)

abstract

  • Untrained rats selectively bred for either high (HCR) or low (LCR) treadmill running capacity previously demonstrated divergent physiological traits as early as the seventh generation (G7). We asked whether continued selective breeding to generation 15 (G15) would further increase the divergence in skeletal muscle capillarity, morphometry, and oxidative capacity seen previously at G7. At G15, mean body weight was significantly lower ( P < 0.001) in the HCR rats ( n = 11; 194 ± 3 g) than in LCR ( n = 12; 259 ± 9 g) while relative medial gastrocnemius muscle mass was not different (0.23 ± 0.01 vs. 0.22 ± 0.01% total body weight). Normoxic (FiO2= 0.21) V̇o2maxwas 50% greater ( P < 0.001) in HCR despite the lower absolute muscle mass, and skeletal muscle O2conductance (measured in hypoxia; FiO2= 0.10) was 49% higher in HCR ( P < 0.001). Muscle oxidative enzyme activities were significantly higher in HCR (citrate synthase: 16.4 ± 0.4 vs. 14.0 ± 0.6; β-hydroxyacyl-CoA dehydrogenase: 5.2 ± 0.2 vs. 4.2 ± 0.2 mmol·kg−1·min−1). HCR rats had ∼36% more total muscle fibers and also 36% more capillaries in the medial gastrocnemius. Because average muscle fiber area was 35% smaller, capillary density was 36% higher in HCR, but capillary-to-fiber ratio was the same. Compared with G7, G15 HCR animals showed 38% greater total fiber number with an additional 25% decrease in mean fiber area. These data suggest that many of the skeletal muscle structural and functional adaptations enabling greater O2utilization in HCR at G7 continue to progress following additional selective breeding for endurance capacity. However, the largest changes at G15 relate to O2delivery to skeletal muscle and not to the capacity of skeletal muscle to use O2.

authors

  • Howlett, Richard A.
  • Kirkton, Scott D.
  • Gonzalez, Norberto C.
  • Wagner, Harrieth E.
  • Britton, Steven L.
  • Koch, Lauren Gerard
  • Wagner, Peter D.

publication date

  • 2009

webpage

published in

number of pages

  • 6

start page

  • 1819

end page

  • 1825

volume

  • 106

issue

  • 6