VASP is a CXCR2-interacting protein that regulates CXCR2-mediated polarization and chemotaxis Article (Web of Science)


  • Chemotaxis regulates the recruitment of leukocytes, which is integral for a number of biological processes and is mediated through the interaction of chemokines with seven transmembrane G-protein-coupled receptors. Several studies have indicated that chemotactic signaling pathways might be activated via G-protein-independent mechanisms, perhaps through novel receptor-interacting proteins. CXCR2 is a major chemokine receptor expressed on neutrophils. We used a proteomics approach to identify unique ligand-dependent CXCR2-interacting proteins in differentiated neutrophil-like HL-60 cells. Using this approach, vasodilator-stimulated phosphoprotein (VASP) was identified as a CXCR2-interacting protein. The interaction between CXCR2 and VASP is direct and enhanced by CXCL8 stimulation, which triggers VASP phosphorylation via PKA- and PKCĪ“-mediated pathways. The interaction between CXCR2 and VASP requires free F-actin barbed ends to recruit VASP to the leading edge. Finally, knockdown of VASP in HL-60 cells results in severely impaired CXCR2-mediated chemotaxis and polarization. These data provide the first demonstration that direct interaction of VASP with CXCR2 is essential for proper CXCR2 function and demonstrate a crucial role for VASP in mediating chemotaxis in leukocytes.


  • Neel, Nicole F.
  • Barzik, Melanie
  • Raman, Dayanidhi
  • Sobolik-Delmaire, Tammy
  • Sai, Jiqing
  • Ham, Amy J.
  • Mernaugh, Raymond L.
  • Gertler, Frank B.
  • Richmond, Ann

publication date

  • 2009

published in

number of pages

  • 12

start page

  • 1882

end page

  • 1894


  • 122


  • 11