High inborn aerobic capacity does not protect the heart following myocardial infarction Article (Web of Science)

abstract

  • Maximal oxygen uptake (V̇o2max) is a strong prognostic marker for morbidity and mortality, but the cardio-protective effect of high inborn V̇o2max remains unresolved. We aimed to investigate whether rats with high inborn V̇o2max yield cardio-protection after myocardial infarction (MI) compared with rats with low inborn V̇o2max. Rats breed for high capacity of running (HCR) or low capacity of running (LCR) were randomized into HCR-SH (sham), HCR-MI, LCR-SH, and LCR-MI. V̇o2max was lower in HCR-MI and LCR-MI compared with respective sham ( P < 0.01), supported by a loss in global cardiac function, assessed by echocardiography. Fura 2-AM loaded cardiomyocyte experiments revealed that HCR-MI and LCR-MI decreased cardiomyocyte shortening (39%, and 34% reduction, respectively, both P < 0.01), lowered Ca2+ transient amplitude (37%, P < 0.01, and 20% reduction, respectively), and reduced sarcoplasmic reticulum (SR) Ca2+ content (both; 20%, P < 0.01) compared with respective sham. Diastolic Ca2+ cycling was impaired in HCR-MI and LCR-MI evidenced by prolonged time to 50% Ca2+ decay that was partly explained by the 47% ( P < 0.01) and 44% ( P < 0.05) decrease in SR Ca2+-ATPase Ca2+ removal, respectively. SR Ca2+ leak increased by 177% in HCR-MI ( P < 0.01) and 67% in LCR-MI ( P < 0.01), which was abolished by inhibition of Ca2+/calmodulin-dependent protein kinase II. This study demonstrates that the effect of MI in HCR rats was similar or even more pronounced on cardiac- and cardiomyocyte contractile function, as well as on Ca2+ handling properties compared with observations in LCR. Thus our data do not support a cardio-protective effect of higher inborn aerobic capacity.

authors

  • Høydal, M. A.
  • Kaurstad, G.
  • Rolim, N. P.
  • Johnsen, A. B.
  • Alves, M.
  • Koch, Lauren Gerard
  • Britton, S. L.
  • Stølen, T. O.
  • Smith, G. L.
  • Wisløff, U.

publication date

  • 2013

webpage

published in

number of pages

  • 7

start page

  • 1788

end page

  • 1795

volume

  • 115

issue

  • 12