Karunarathne, Welivitiya K Ajith

Welivitiya K Ajith Karunarathne
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Positions

Associate Professor, Chemistry & Biochemistry , College of Natural Sciences and Mathematics

Contact Info

Ajith.Karunarathne@utoledo.edu

http://www.utoledo.edu/nsm/chemistry/people/Webpages/Karunarathne.html

selected publications

Article (Faculty180)
- 2021
  
  A short C-terminal peptide in Gγ regulates Gβγ signaling efficacy.. Molecular Biology of the Cell.. 32:1446-1458.
- 2021
  
  Dissociation of the G protein βγ from the Gq-PLCβ complex partially attenuates PIP2 hydrolysis.. Journal of Biological Chemistry.
- 2021
  
  Subtype-dependent regulation of Gβγ signalling.. Cellular Signalling.
- 2020
  
  A C-peptide complex with albumin and Zn(2+) increases measurable GLUT1 levels in membranes of human red blood cells.. Nature Scientific Reports .
- 2020
  
  Blue light-triggered photochemistry and cytotoxicity of retinal. Cellular Signalling. 69.
- 2020
  
  Microcystins: Biogenesis, Toxicity, Analysis, and Control. LID - 10.1021/acs.chemrestox.0c00164 [doi]. Chemical Research in Toxicology. 34:22.
- 2019
  
  Statins Perturb Gβγ Signaling and Cell Behavior in a Gγ Subtype Dependent Manner.. Molecular Pharmacology. 95:361-375.
- 2019
  
  G protein alpha q exerts expression level-dependent distinct signaling paradigms. Cellular Signalling. 58:34-43.
- 2019
  
  Optical approaches for single-cell and subcellular analysis of GPCRâG protein signaling. Analytical and Bioanalytical Chemistry. 411:4481-4508.
- 2018
  
  Blue light excited retinal intercepts cellular signaling. Scientific Reports. 8:10207.
- 2018
  
  Blue light-excited retinal delocalizes plasma membrane bound PIP2 and triggers stress responses challenging the cellular integrity. The FASEB Journal. 32:533.36-533.36.
- 2018
  
  Confocal Imaging and k-Means Clustering of GABAB and mGluR Mediated Modulation of Ca2+ Spiking in Hippocampal Neurons. ACS Chemical Neuroscience.
- 2018
  
  G protein γ (Gγ) subtype dependent targeting of GRK2 to M3 receptor by Gβγ. Biochemical and Biophysical Research Communications. 503:165-170.
- 2018
  
  Gγ identity dictates efficacy of Gβγ signaling and macrophage migration.. Journal of Biological Chemistry. 293:2974-2989.
- 2018
  
  Melanopsin (Opn4) utilizes Gαi and Gβγ as major signal transducers. Journal of Cell Science. 131.
- 2017
  
  Comparison of Calcium Dynamics and Specific Features for G Protein–Coupled Receptor–Targeting Drugs Using Live Cell Imaging and Automated Analysis . SLAS DISCOVERY: Advancing Life Sciences R&D. 22:848-858.
- 2017
  
  Two independent but synchronized Gβγ subunit–controlled pathways are essential for trailing-edge retraction during macrophage migration. Journal of Biological Chemistry. 292:17482-17495.
- 2016
  
  Facilitation of TRPV4 by TRPV1 is required for itch transmission in some sensory neuron populations. Science Signaling. 9:ra71.
- 2016
  
  Reversible G Protein βγ9 Distribution-Based Assay Reveals Molecular Underpinnings in Subcellular, Single-Cell, and Multicellular GPCR and G Protein Activity. Analytical Chemistry. 88:11450-11459.
- 2015
  
  Subcellular optogenetics – controlling signaling and single-cell behavior. Journal of Cell Science. 128:15.
- 2015
  
  Subcellular optogenetics--controlling signaling and single-cell behavior. J Cell Sci. jcs.
- 2014
  
  A G-protein subunit translocation embedded network motif underlies GPCR regulation of calcium oscillations. Biophysical journal. 107:242.
- 2014
  
  Descending Control of Itch Transmission by the Serotonergic System via 5-HT1A-Facilitated GRP-GRPR Signaling. Neuron. 84:821-834.
- 2014
  
  Descending control of itch transmission by the serotonergic system via 5-HT1A-facilitated GRP-GRPR signaling. Neuron. 84:821.
- 2013
  
  Optical control demonstrates switch-like PIP3 dynamics underlying the initiation of immune cell migration. Proceedings of the National Academy of Sciences. 201220755.
- 2013
  
  Optically triggering spatiotemporally confined GPCR activity in a cell and programming neurite initiation and extension. Proceedings of the National Academy of Sciences. 201220697.
- 2013
  
  The structure of dynamic GPCR signaling networks. Wiley Interdisciplinary Reviews: Systems Biology and Medicine. 6:115.
- 2012
  
  All G protein betagamma complexes are capable of translocation on receptor activation. Biochemical and biophysical research communications. 421:605.
- 2012
  
  G-protein signaling leverages subunit-dependent membrane affinity to differentially control βγ translocation to intracellular membranes. Proceedings of the National Academy of Sciences. 109:E3568.
- 2012
  
  Measuring P2X1 receptor activity in washed platelets in the absence of exogenous apyrase. Analytical Methods. 4:101.
- 2011
  
  Alteration of Golgi structure in senescent cells and its regulation by a G protein gamma subunit. Cellular signalling. 23:785.
- 2010
  
  Hydroxyurea stimulates the release of ATP from rabbit erythrocytes through an increase in calcium and nitric oxide production. European journal of pharmacology. 645:32.
- 2010
  
  Regulation of Golgi structure and secretion by receptor-induced G protein beta gamma complex translocation. Proceedings of the National Academy of Sciences. 107:11417.
- 2010
  
  Use of the red blood cell as a simple drug target and diagnostic by manipulating and monitoring its ability to release adenosine triphosphate (ATP). Pure and Applied Chemistry. 82:1623.
- 2009
  
  The dual nature of extracellular ATP as a concentration-dependent platelet P2X1 agonist and antagonist. Integrative Biology. 1:655.
- 2008
  
  Metal-activated C-peptide facilitates glucose clearance and the release of a nitric oxide stimulus via the GLUT1 transporter. Diabetologia. 51:175.
- 2007
  
  Fluorescence determination of nitric oxide production in stimulated and activated platelets. Analytical chemistry. 79:2421.
- 2007
  
  Red blood cell stimulation of platelet nitric oxide production indicated by quantitative monitoring of the communication between cells in the bloodstream. Analytical chemistry. 79:5133.
Article (Web of Science)
- 2021
  
  Subtype-dependent regulation of G beta gamma signalling. CELLULAR SIGNALLING. 82.
  Full Text via DOI: 10.1016/j.cellsig.2021.109947 PMID: 33582184
- 2021
  
  Dissociation of the G protein beta gamma from the Gq-PLC beta complex partially attenuates PIP2 hydrolysis. JOURNAL OF BIOLOGICAL CHEMISTRY. 296.
  Full Text via DOI: 10.1016/j.jbc.2021.100702 PMID: 33901492
- 2021
  
  A short C-terminal peptide in Gγ regulates Gβγ signaling efficacy. MOLECULAR BIOLOGY OF THE CELL. mbc.E20-11-0750.
  Full Text via DOI: 10.1091/mbc.e20-11-0750
- 2020
  
  A C-peptide complex with albumin and Zn2+ increases measurable GLUT1 levels in membranes of human red blood cells. SCIENTIFIC REPORTS. 10.
  Full Text via DOI: 10.1038/s41598-020-74527-6 PMID: 33060722
- 2020
  
  Blue light-triggered photochemistry and cytotoxicity of retinal. CELLULAR SIGNALLING. 69.
  Full Text via DOI: 10.1016/j.cellsig.2020.109547 PMID: 31982549
- 2019
  
  G protein alpha q exerts expression level-dependent distinct signaling paradigms. CELLULAR SIGNALLING. 58:34-43.
  Full Text via DOI: 10.1016/j.cellsig.2019.02.006 PMID: 30849518
- 2019
  
  Statins Perturb G beta gamma Signaling and Cell Behavior in a G gamma Subtype Dependent Manner. MOLECULAR PHARMACOLOGY. 95:361-375.
  Full Text via DOI: 10.1124/mol.118.114710
- 2018
  
  Confocal Imaging and k-Means Clustering of GABA(B) and mGluR Mediated Modulation of Ca2+ Spiking in Hippocampal Neurons. ACS CHEMICAL NEUROSCIENCE. 9:3094-3107.
  Full Text via DOI: 10.1021/acschemneuro.8b00297
- 2018
  
  G protein gamma (G gamma) subtype dependent targeting of GRK2 to M3 receptor by G beta gamma. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 503:165-170.
  Full Text via DOI: 10.1016/j.bbrc.2018.05.204
- 2018
  
  Blue light excited retinal intercepts cellular signaling. SCIENTIFIC REPORTS. 8.
  Full Text via DOI: 10.1038/s41598-018-28254-8
- 2018
  
  Melanopsin (Opn4) utilizes G alpha(i) and G beta gamma as major signal transducers. JOURNAL OF CELL SCIENCE. 131.
  Full Text via DOI: 10.1242/jcs.212910
- 2018
  
  G gamma identity dictates efficacy of G beta gamma signaling and macrophage migration. JOURNAL OF BIOLOGICAL CHEMISTRY. 293:2974-2989.
  Full Text via DOI: 10.1074/jbc.RA117.000872
- 2018
  
  Gγ identity dictates efficacy of Gβγ signaling and macrophage migration. JOURNAL OF BIOLOGICAL CHEMISTRY. 293:2974-2989.
  Full Text via DOI: 10.1074/jbc.ra117.000872
- 2017
  
  Two independent but synchronized G beta gamma subunit-controlled pathways are essential for trailing-edge retraction during macrophage migration. JOURNAL OF BIOLOGICAL CHEMISTRY. 292:17482-17495.
  Full Text via DOI: 10.1074/jbc.M117.787838
- 2017
  
  Comparison of Calcium Dynamics and Specific Features for G Protein-Coupled Receptor-Targeting Drugs Using Live Cell Imaging and Automated Analysis. SLAS DISCOVERY. 22:848-858.
  Full Text via DOI: 10.1177/2472555217693378
- 2017
  
  Two independent but synchronized Gβγ subunit–controlled pathways are essential for trailing-edge retraction during macrophage migration. JOURNAL OF BIOLOGICAL CHEMISTRY. 292:17482-17495.
  Full Text via DOI: 10.1074/jbc.m117.787838
- 2016
  
  Reversible G Protein beta gamma 9 Distribution-Based Assay Reveals Molecular Underpinnings in Subcellular, Single-Cell, and Multicellular GPCR and G Protein Activity. ANALYTICAL CHEMISTRY. 88:11450-11459.
  Full Text via DOI: 10.1021/acs.analchem.6b02512
- 2016
  
  Facilitation of TRPV4 by TRPV1 is required for itch transmission in some sensory neuron populations. SCIENCE SIGNALING. 9.
  Full Text via DOI: 10.1126/scisignal.aaf1047
- 2015
  
  Subcellular optogenetics - controlling signaling and single-cell behavior. JOURNAL OF CELL SCIENCE. 128:15-25.
  Full Text via DOI: 10.1242/jcs.154435
- 2014
  
  A G-Protein Subunit Translocation Embedded Network Motif Underlies GPCR Regulation of Calcium Oscillations. BIOPHYSICAL JOURNAL. 107:242-254.
  Full Text via DOI: 10.1016/j.bpj.2014.05.020
- 2014
  
  Descending Control of Itch Transmission by the Serotonergic System via 5-HT1A-Facilitated GRP-GRPR Signaling. Neuron. 84:821-834.
  Full Text via DOI: 10.1016/j.neuron.2014.10.003
- 2014
  
  The structure of dynamic GPCR signaling networks. Wiley Interdisciplinary Reviews: Systems Biology and Medicine. 6:115-123.
  Full Text via DOI: 10.1002/wsbm.1249
- 2013
  
  Optical control demonstrates switch-like PIP3 dynamics underlying the initiation of immune cell migration. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 110.
  Full Text via DOI: 10.1073/pnas.1220755110
- 2013
  
  Optically triggering spatiotemporally confined GPCR activity in a cell and programming neurite initiation and extension. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 110.
  Full Text via DOI: 10.1073/pnas.1220697110
- 2012
  
  All G protein βγ complexes are capable of translocation on receptor activation. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 421:605-611.
  Full Text via DOI: 10.1016/j.bbrc.2012.04.054
- 2012
  
  G-protein signaling leverages subunit-dependent membrane affinity to differentially control βγ translocation to intracellular membranes. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 109.
  Full Text via DOI: 10.1073/pnas.1205345109
- 2012
  
  Measuring P2X1 receptor activity in washed platelets in the absence of exogenous apyrase. ANALYTICAL METHODS. 4:101-105.
  Full Text via DOI: 10.1039/c1ay05530e
- 2011
  
  Alteration of Golgi structure in senescent cells and its regulation by a G protein γ subunit. CELLULAR SIGNALLING. 23:785-793.
  Full Text via DOI: 10.1016/j.cellsig.2011.01.001
- 2010
  
  Hydroxyurea stimulates the release of ATP from rabbit erythrocytes through an increase in calcium and nitric oxide production. EUROPEAN JOURNAL OF PHARMACOLOGY. 645:32-38.
  Full Text via DOI: 10.1016/j.ejphar.2010.07.012
- 2010
  
  Regulation of Golgi structure and secretion by receptor-induced G protein βγ complex translocation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 107:11417-11422.
  Full Text via DOI: 10.1073/pnas.1003042107
- 2010
  
  Use of the red blood cell as a simple drug target and diagnostic by manipulating and monitoring its ability to release adenosine triphosphate (ATP). PURE AND APPLIED CHEMISTRY. 82:1623-1634.
  Full Text via DOI: 10.1351/pac-con-10-02-10
- 2009
  
  The dual nature of extracellular ATP as a concentration-dependent platelet P2X1 agonist and antagonist. Integrative Biology. 1:655.
  Full Text via DOI: 10.1039/b909873a
- 2007
  
  Metal-activated C-peptide facilitates glucose clearance and the release of a nitric oxide stimulus via the GLUT1 transporter. DIABETOLOGIA. 51:175-182.
  Full Text via DOI: 10.1007/s00125-007-0853-3
Book Chapter (Web of Science)
- 2018
  
  Regulation of G Protein beta gamma Signaling. G PROTEIN-COUPLED RECEPTORS: EMERGING PARADIGMS IN ACTIVATION, SIGNALING AND REGULATION, PT B. 133-191.
  Full Text via DOI: 10.1016/bs.ircmb.2018.02.008
- 2017
  
  Measurement of GPCR-G protein activity in living cells. G PROTEIN-COUPLED RECEPTORS, 2ND EDITION, PT A. 1-25.
  Full Text via DOI: 10.1016/bs.mcb.2017.07.008
Chapter (Faculty180)
- 2018
  
  Chapter Five - Regulation of G Protein βγ Signaling. G Protein-Coupled Receptors: Emerging Paradigms in Activation, Signaling and Regulation Part B. Academic Press. 133-191.
- 2017
  
  Chapter 1 - Measurement of GPCR-G protein activity in living cells. G Protein-Coupled Receptors Part A. Academic Press. 1-25.
Conference Poster (Faculty180)
Correction (Web of Science)
- 2020
  
  Blue light-triggered photochemistry and cytotoxicity of retinal cellular signalling (vol 69, 109,547, 2020). CELLULAR SIGNALLING.
  Full Text via DOI: 10.1016/j.cellsig.2020.109667 PMID: 32418778
Presentation (Faculty180)
Press (Faculty180)
- 2018
  
  Press and media reports on our published research in Blue Light - Retinal and Cytotoxicity. USA TODAY, FORBES, THE TELEGRAPH, NEWSWEEK, THE GUARDIAN ETC...
Proceedings (Faculty180)
- 2015
  
  Segmentation of neuron and measurement of optically programed neurite growth: Fast automation via Bayesian thresholding. 996-999.
- 2009
  
  Platelet Homeostasis and the P2X (1) Receptor: The Dual Nature of Extracellular ATP as a Concentration-Dependent Platelet Agonist and Antagonist
Proceedings Paper (Web of Science)
- 2021
  
  Heterogeneity in Neuronal Calcium Spike Trains based on Empirical Distance
  Full Text via DOI: 10.1109/ner49283.2021.9441175
- 2021
  
  Information Content in Neuronal Calcium Spike Trains: Entropy Rate Estimation based on Empirical Probabilities
  Full Text via DOI: 10.1109/iecbes48179.2021.9398798
- 2015
  
  Segmentation of Neuron and Measurement of Optically Programed Neurite Growth: Fast Automation via Bayesian Thresholding. 2015 7TH INTERNATIONAL IEEE/EMBS CONFERENCE ON NEURAL ENGINEERING (NER). 996-999.
Review Article (Web of Science)
- 2020
  
  Microcystins: Biogenesis, Toxicity, Analysis, and Control. CHEMICAL RESEARCH IN TOXICOLOGY. 2225-2246.
  Full Text via DOI: 10.1021/acs.chemrestox.0c00164 PMID: 32614166
- 2019
  
  Optical approaches for single-cell and subcellular analysis of GPCR-G protein signaling. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 4481-4508.
  Full Text via DOI: 10.1007/s00216-019-01774-6 PMID: 30927013

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Press and media reports on our published research in Blue Light - Retinal and Cytotoxicity Press (Faculty180)

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Welivitiya K Ajith Karunarathne

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