Timothy Mueser
Contact Info
Publications
selected publications
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Article (Faculty180)
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2024
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2023Equilibration of precipitants in a counter-diffusion apparatus for protein crystallization. JOURNAL OF APPLIED CRYSTALLOGRAPHY. 56:1057-1065.
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2022An N-H-N low-barrier hydrogen bond preorganizes the catalytic site of aspartate aminotransferase to facilitate the second half-reaction. CHEMICAL SCIENCE. 13:10057-10065.
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2022Imaging active site chemistry and protonation states: NMR crystallography of the tryptophan synthase alpha-aminoacrylate intermediate. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 119.
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2022
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2020Pyridoxal 5 '-phosphate dependent reactions: Analyzing the mechanism of aspartate aminotransferase. NEUTRON CRYSTALLOGRAPHY IN STRUCTURAL BIOLOGY. 634:333-359.
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2020Substrate Binding Stiffens Aspartate Aminotransferase by Altering the Enzyme Picosecond Vibrational Dynamics. ACS Omega. 5:18787–18797.
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2018Hyperconjugation Promotes Catalysis in a Pyridoxal 5 '-Phosphate-Dependent Enzyme. Acs Catalysis. 8:6733-6737.
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2017Direct evidence that an extended hydrogen-bonding network influences activation of pyridoxal 5-phosphate in aspartate aminotransferase. Journal of Biological Chemistry. 292:5970-5980.
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2017Direct visualization of critical hydrogen atoms in a pyridoxal 5'-phosphate enzyme. Nature Communications. 8:DOI: 10.1038/s41467.
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2016Visualizing the Bohr effect in hemoglobin: neutron structure of equine cyanomethemoglobin in the R state and comparison with human deoxyhemoglobin in the T state. Acta Crystallogr D Struct Biol. 72:892-903.
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2013Thermodynamic and structural analysis of DNA damage architectures related to replication. J Nucleic Acids. 2013:867957.
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2013Thermodynamic and structural analysis of DNA damage architectures related to replication. J. Nucleic Acids. 2013.
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2012Complete sequence determination of hemoglobin from endangered feline species using a combined ESI-MS and X-ray crystallography approach. International Journal of Mass Spectrometry. 312:70-77.
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2011Allosteric interaction of nucleotides and tRNA(ala) with E. coli alanyl-tRNA synthetase. Biochemistry. 50:9886-900.
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2010Hemoglobin redux: combining neutron and X-ray diffraction with mass spectrometry to analyse the quaternary state of oxidized hemoglobins. Acta Crystallogr D Biol Crystallogr. 66:1249-56.
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2010Preliminary neutron and X-ray crystallographic studies of equine cyanomethemoglobin. Acta Crystallogr Sect F Struct Biol Cryst Commun. 66:474-7.
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2008Optimization of buffer solutions for protein crystallization. Acta Crystallogr D Biol Crystallogr. 64:506-14.
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2007
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2006Architecture and function of the phage T4 replication fork. FASEB Journal. 20.
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2006Assessment of a preliminary solubility screen to improve crystallization trials: uncoupling crystal condition searches. Acta Crystallogr D Biol Crystallogr. 62:833-42.
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2006Purification, crystallization and preliminary X-ray diffraction of human S100A15. Acta Crystallogr Sect F Struct Biol Cryst Commun. 62:467-70.
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2005Rapid preparation of custom grid screens for crystal growth optimization. Journal of Applied Crystallography. 38:847-850.
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2004A preliminary solubility screen used to improve crystallization trials: crystallization and preliminary X-ray structure determination of Aeropyrum pernix flap endonuclease-1. Acta Crystallogr D Biol Crystallogr. 60:1674-8.
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2004Crystallography and mutagenesis point to an essential role for the N-terminus of human mitochondrial ClpP. J Struct Biol. 148:338-52.
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2001Bacteriophage T4 gene 41 helicase and gene 59 helicase-loading protein: a versatile couple with roles in replication and recombination. Proc Natl Acad Sci U S A. 98:8312-8.
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2000Crystal structure of an active two-domain derivative of Rous sarcoma virus integrase. J Mol Biol. 296:535-48.
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2000Interface sliding as illustrated by the multiple quaternary structures of liganded hemoglobin. Biochemistry. 39:15353-64.
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1999The crystal structure of the SIV gp41 ectodomain at 1.47 A resolution. J Struct Biol. 126:131-44.
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1998
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1990Quantitation of the Binding of Oxygen to Human Hemoglobin. Biophysical Journal. 57:A231-A231.
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1986
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1983Neither REM sleep deprivation nor rebound influences 3H-diazepam binding n rat brain. Physiol Behav. 31:237-9.
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Article (Web of Science)
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2022Imaging active site chemistry and protonation states: NMR crystallography of the tryptophan synthase alpha-aminoacrylate intermediate. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 119.Full Text via DOI: 10.1073/pnas.2109235119 PMID: 34996869
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2020Substrate Binding Stiffens Aspartate Aminotransferase by Altering the Enzyme Picosecond Vibrational Dynamics. ACS OMEGA. 5:18787-18797.Full Text via DOI: 10.1021/acsomega.0c01900 PMID: 32775880
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2018Hyperconjugation Promotes Catalysis in a Pyridoxal 5 '-Phosphate-Dependent Enzyme. ACS CATALYSIS. 8:6733-6737.Full Text via DOI: 10.1021/acscatal.8b01911
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2018Neutron diffraction studies of pyridoxal-5′-phosphate-dependent enzymes. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES. 74:a36-a36.Full Text via DOI: 10.1107/s0108767318099634
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2017Direct visualization of critical hydrogen atoms in a pyridoxal 5 '-phosphate enzyme. NATURE COMMUNICATIONS. 8.Full Text via DOI: 10.1038/s41467-017-01060-y
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2017Direct evidence that an extended hydrogen-bonding network influences activation of pyridoxal 5-phosphate in aspartate aminotransferase. JOURNAL OF BIOLOGICAL CHEMISTRY. 292:5970-5980.Full Text via DOI: 10.1074/jbc.M116.774588
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2017Direct evidence that an extended hydrogen-bonding network influences activation of pyridoxal 5′-phosphate in aspartate aminotransferase. JOURNAL OF BIOLOGICAL CHEMISTRY. 292:5970-5980.Full Text via DOI: 10.1074/jbc.m116.774588
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2016Visualizing the Bohr effect in hemoglobin: neutron structure of equine cyanomethemoglobin in the R state and comparison with human deoxyhemoglobin in the T state. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY. 72:892-903.Full Text via DOI: 10.1107/S2059798316009049
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2016Visualizing the Bohr effect in hemoglobin: neutron structure of equine cyanomethemoglobin in the R state and comparison with human deoxyhemoglobin in the T state. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY. 72:892-903.Full Text via DOI: 10.1107/s2059798316009049
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2013Thermodynamic and Structural Analysis of DNA Damage Architectures Related to Replication. Journal of Nucleic Acids. 2013:1-10.Full Text via DOI: 10.1155/2013/867957
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2012Crystal Structure of 12-Lipoxygenase Catalytic-Domain-Inhibitor Complex Identifies a Substrate-Binding Channel for Catalysis. STRUCTURE. 20:1490-1497.Full Text via DOI: 10.1016/j.str.2012.06.003
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2012Models for the Binary Complex of Bacteriophage T4 Gp59 Helicase Loading Protein GP32 SINGLE-STRANDED DNA-BINDING PROTEIN AND TERNARY COMPLEX WITH PSEUDO-Y JUNCTION DNA. JOURNAL OF BIOLOGICAL CHEMISTRY. 287:18608-18617.Full Text via DOI: 10.1074/jbc.M111.333476
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2012Mutational Analysis of the T4 Gp59 Helicase Loader Reveals Its Sites for Interaction with Helicase, Single-stranded Binding Protein, and DNA. JOURNAL OF BIOLOGICAL CHEMISTRY. 287:18596-18607.Full Text via DOI: 10.1074/jbc.M111.332080
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2012Complete sequence determination of hemoglobin from endangered feline species using a combined ESI-MS and X-ray crystallography approach. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY. 312:70-77.Full Text via DOI: 10.1016/j.ijms.2011.06.001
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2012Models for the Binary Complex of Bacteriophage T4 Gp59 Helicase Loading Protein. JOURNAL OF BIOLOGICAL CHEMISTRY. 287:18608-18617.Full Text via DOI: 10.1074/jbc.m111.333476
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2012Mutational Analysis of the T4 Gp59 Helicase Loader Reveals Its Sites for Interaction with Helicase, Single-stranded Binding Protein, and DNA. JOURNAL OF BIOLOGICAL CHEMISTRY. 287:18596-18607.Full Text via DOI: 10.1074/jbc.m111.332080
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2011Allosteric Interaction of Nucleotides and tRNA(ala) with E. coli Alanyl-tRNA Synthetase. BIOCHEMISTRY. 50:9886-9900.Full Text via DOI: 10.1021/bi2012004
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2010Hemoglobin redux: combining neutron and X-ray diffraction with mass spectrometry to analyse the quaternary state of oxidized hemoglobins. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY. 66:1249-1256.Full Text via DOI: 10.1107/S090744491002545X
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2010Preliminary neutron and X-ray crystallographic studies of equine cyanomethemoglobin. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS. 66:474-477.Full Text via DOI: 10.1107/S1744309110007840
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2010Hemoglobin redux: combining neutron and X-ray diffraction with mass spectrometry to analyse the quaternary state of oxidized hemoglobins. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY. 66:1249-1256.Full Text via DOI: 10.1107/s090744491002545x
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2010Preliminary neutron and X-ray crystallographic studies of equine cyanomethemoglobin. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS. 66:474-477.Full Text via DOI: 10.1107/s1744309110007840
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2008Optimization of buffer solutions for protein crystallization. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY. 64:506-514.Full Text via DOI: 10.1107/S0907444908004265
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2008Optimization of buffer solutions for protein crystallization. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY. 64:506-514.Full Text via DOI: 10.1107/s0907444908004265
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2007Crystal structure of bacteriophage T4 5' nuclease in complex with a branched DNA reveals how flap endonuclease-1 family nucleases bind their substrates. JOURNAL OF BIOLOGICAL CHEMISTRY. 282:31713-31724.Full Text via DOI: 10.1074/jbc.M703209200
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2007A three-dimensional model of the human transglutaminase 1: insights into the understanding of lamellar ichthyosis. JOURNAL OF MOLECULAR MODELING. 13:233-246.Full Text via DOI: 10.1007/s00894-006-0144-9
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2007Crystal Structure of Bacteriophage T4 5′ Nuclease in Complex with a Branched DNA Reveals How Flap Endonuclease-1 Family Nucleases Bind Their Substrates. JOURNAL OF BIOLOGICAL CHEMISTRY. 282:31713-31724.Full Text via DOI: 10.1074/jbc.m703209200
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2006Assessment of a preliminary solubility screen to improve crystallization trials: uncoupling crystal condition searches. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY. 62:833-842.Full Text via DOI: 10.1107/S0907444906018385
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2006Purification, crystallization and preliminary X-ray diffraction of human S100A15. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS. 62:467-470.Full Text via DOI: 10.1107/S1744309106012838
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2006Assessment of a preliminary solubility screen to improve crystallization trials: uncoupling crystal condition searches. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY. 62:833-842.Full Text via DOI: 10.1107/s0907444906018385
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2006Purification, crystallization and preliminary X-ray diffraction of human S100A15. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS. 62:467-470.Full Text via DOI: 10.1107/s1744309106012838
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2005Rapid preparation of custom grid screens for crystal growth optimization. JOURNAL OF APPLIED CRYSTALLOGRAPHY. 38:847-850.Full Text via DOI: 10.1107/S0021889805019953
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2005Rapid preparation of custom grid screens for crystal growth optimization. JOURNAL OF APPLIED CRYSTALLOGRAPHY. 38:847-850.Full Text via DOI: 10.1107/s0021889805019953
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2004A preliminary solubility screen used to improve crystallization trials: crystallization and preliminary X-ray structure determination ofAeropyrum pernixflap endonuclease-1. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY. 60:1674-1678.Full Text via DOI: 10.1107/s090744490401844x
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2004Bacteriophage T4 32 Protein Is Required for Helicase-dependent Leading Strand Synthesis When the Helicase Is Loaded by the T4 59 Helicase-loading Protein. JOURNAL OF BIOLOGICAL CHEMISTRY. 279:12067-12075.Full Text via DOI: 10.1074/jbc.m313840200
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2004Crystallography and mutagenesis point to an essential role for the N-terminus of human mitochondrial ClpP. Journal of Structural Biology. 148:338-352.Full Text via DOI: 10.1016/j.jsb.2004.07.004
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2004Mutations of Bacteriophage T4 59 Helicase Loader Defective in Binding Fork DNA and in Interactions with T4 32 Single-stranded DNA-binding Protein. JOURNAL OF BIOLOGICAL CHEMISTRY. 279:25721-25728.Full Text via DOI: 10.1074/jbc.m402128200
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2001Bacteriophage T4 gene 41 helicase and gene 59 helicase-loading protein: A versatile couple with roles in replication and recombination. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 98:8312-8318.Full Text via DOI: 10.1073/pnas.121009398
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2000Bacteriophage T4 gene 59 helicase assembly protein binds replication fork DNA. The 1.45 Å resolution crystal structure reveals a novel α-helical two-domain fold 1 1Edited by P. E. Wright. JOURNAL OF MOLECULAR BIOLOGY. 296:597-612.Full Text via DOI: 10.1006/jmbi.1999.3438
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2000Crystal structure of an active two-domain derivative of rous sarcoma virus integrase 1 1Edited by I. A. Wilson. JOURNAL OF MOLECULAR BIOLOGY. 296:535-548.Full Text via DOI: 10.1006/jmbi.1999.3463
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2000Interaction of the bacteriophage T4 gene 59 helicase loading protein and gene 41 helicase with each other and with fork, flap, and cruciform DNA.. JOURNAL OF BIOLOGICAL CHEMISTRY. 275:27145-54.Full Text via DOI: 10.1074/jbc.m003808200 PMID: 10871615
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2000Interface Sliding As Illustrated by the Multiple Quaternary Structures of Liganded Hemoglobin,. BIOCHEMISTRY. 39:15353-15364.Full Text via DOI: 10.1021/bi0012944
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1999The Crystal Structure of the SIV gp41 Ectodomain at 1.47 Å Resolution. Journal of Structural Biology. 126:131-144.Full Text via DOI: 10.1006/jsbi.1999.4116
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1998Purification of Recombinant Rous Sarcoma Virus Integrase Possessing Physical and Catalytic Properties Similar to Virion-Derived Integrase. PROTEIN EXPRESSION AND PURIFICATION. 14:167-177.Full Text via DOI: 10.1006/prep.1998.0954
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1997Avian Retrovirus U3 and U5 DNA Inverted Repeats. JOURNAL OF BIOLOGICAL CHEMISTRY. 272:23938-23945.Full Text via DOI: 10.1074/jbc.272.38.23938
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1996High-resolution structures of liganded canine and feline hemoglobin reveal an alternative quaternary conformation. ACTA CRYSTALLOGRAPHICA SECTION A. 52:C245-C245.Full Text via DOI: 10.1107/s010876739608960x
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1996Structure of Bacteriophage T4 RNase H, a 5′ to 3′ RNA–DNA and DNA–DNA Exonuclease with Sequence Similarity to the RAD2 Family of Eukaryotic Proteins. CELL. 85:1101-1112.Full Text via DOI: 10.1016/s0092-8674(00)81310-0
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1993Synthesis of dansyl ribonucleotides and their use in steady-state fluorescence anisotropy studies of nucleotide binding by initiation factor-2 (eif-2) and histone hi. International Journal of Biochemistry. 25:1689-1696.Full Text via DOI: 10.1016/0020-711x(93)90529-n
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1991Use of dual wavelength spectrophotometry and continuous enzymatic depletion of oxygen for determination of the oxygen binding constants of hemoglobin. ANALYTICAL BIOCHEMISTRY. 197:231-246.Full Text via DOI: 10.1016/0003-2697(91)90383-5
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1986The association of NADPH with the guanine nucleotide exchange factor from rabbit reticulocytes: a role of pyridine dinucleotides in eukaryotic polypeptide chain initiation.. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 83:6746-6750.Full Text via DOI: 10.1073/pnas.83.18.6746
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1983Neither REM sleep deprivation nor rebound influences 3H-diazepam binding in rat brain. PHYSIOLOGY & BEHAVIOR. 31:237-239.Full Text via DOI: 10.1016/0031-9384(83)90126-9
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Book Chapter (Web of Science)
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2020Pyridoxal 5 '-phosphate dependent reactions: Analyzing the mechanism of aspartate aminotransferase. NEUTRON CRYSTALLOGRAPHY IN STRUCTURAL BIOLOGY. 333-359.Full Text via DOI: 10.1016/bs.mie.2020.01.009 PMID: 32093839
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Chapter (Faculty180)
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2011Alkylation chemotherapy: Mechanistic potential and pitfalls.. Mutations: Types, Harmful Mutations and Beneficial Mutations. Advances in Genetics Research. Nova Science Publishers, Inc.. Alkylation chemotherapy: Mechanistic potential and pitfalls..
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Meeting Abstract (Web of Science)
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2020HP-Bio: high-pressure BioSAXS for deep life and extreme biophysics. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES. a136-a136.Full Text via DOI: 10.1107/s0108767320098645
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2019Neutron diffraction studies of PLP-dependent enzymes. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES. a131-a131.Full Text via DOI: 10.1107/s0108767319098684
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2019Use of microgravity for the preparation of the large-volume crystals required in neutron diffraction studies. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES. a267-a267.Full Text via DOI: 10.1107/s0108767319097381
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2018Crystal growth in microgravity for neutron diffraction studies. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES. a293-a293.Full Text via DOI: 10.1107/s0108767318097064
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2017Direct observation of protonation states in a PLP-dependent enzyme by neutron crystallography. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES. a26-a26.Full Text via DOI: 10.1107/s0108767317099731
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2015Visualization of Pronation States in PLP Dependent Enzymes. FASEB JOURNAL.
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2013Oxidative DNA damage in replication relevant architectures. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
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2012Kinetics of Biotin Derivatives Binding to Avidin and Streptavidin. BIOPHYSICAL JOURNAL. 217A-217A.
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2012Kinetics of Biotin Derivatives Binding to Avidin and Streptavidin. BIOPHYSICAL JOURNAL. 217a.Full Text via DOI: 10.1016/j.bpj.2011.11.1189
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2010Creation, analysis and selective oxidation of modified replication related DNA architectures. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
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2006Architecture and function of the phage T4 replication fork. FASEB JOURNAL. A420-A420.
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Review Article (Web of Science)
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2010Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives. VIROLOGY JOURNAL.Full Text via DOI: 10.1186/1743-422X-7-359
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2010Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives. VIROLOGY JOURNAL. 359.Full Text via DOI: 10.1186/1743-422x-7-359
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Contact
full name
- Timothy Mueser
visualizations
Cumulative publications in Scholars@UToledo