Maria Diakonova
- Professor (tenured), Biological Sciences , College of Natural Sciences and Mathematics
Overview
overview
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I graduated from the Leningrad (now St.-Petersburg) State University, USSR (now Russia) in 1984 with a Master in Science degree. In the Russian Academic system it is typical to work for several years between finishing university and starting a Ph.D. program. After the University I was invited to join the staff of the Russian Cell Culture Collection, operated in the Institute of Cytology, Russian Academy of Science. In 1988 I started my graduate school in the Laboratory of Cell Cycle Physiology of the Institute of Cytology. During my time in graduate school I was involved in several collaborative projects outside of Russia. In 1991 I worked for 6 in the laboratory of Dr. Guillouzo in Rennes, France. In 1992 I worked at the University of Utrecht, Netherlands, under supervision of Dr. Boonstra. I graduated in 1993 and started my first postdoctoral training at the European Molecular Biology Laboratory (EMBL, Heidelberg, Germany, 1995 – 1997) with Dr. Griffiths. Upon coming to the U.S.A., I first joined the laboratory of Dr. Swanson at the University of Michigan, Ann Arbor (1997-1999) and the laboratory of Dr. Carter-Su (1999-2002) after that. I was promoted to the Assistant Research Professor position at the Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, in 2002, where I started my own lab.
In 2006 I was invited to the Biological Science Department of the University of Toledo as Assistant Professor and was promoted to Associate Professor with tenure in 2012. Since 2006 I have published 16 papers in reputable journals on a role of hormones prolactin and estrogen in breast cancer progression, and served as the main editor of “Recent Advances in Prolactin Research” book (Springer, 2015). In addition to research, I have acquired extensive teaching experience at undergraduate and graduate levels. I have taught undergraduate courses, “Biological Literature and Communication – WAC” (BIOL 4700) and “Human Physiology” (BIOL 3070). I have also taught a graduate course, “Advanced Cell Biology” (BIOL 6090/8090). In addition to my classroom teaching, I have served as a major professor for 4 Ph.D. students (3 of whom were undergraduate students in my lab) and 3 Master students to degree completion.
Publications
selected publications
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Article (Faculty180)
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2024Prolactin-induced tyrosyl phosphorylation of PAK1 facilitates epithelial-mesenchymal transition. . microPublication Biology. .
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2024Prolactin-activated PAK1 potentiates estrogen response to breast cancer cell epithelial-mesenchymal transition, migration and invasion. . microPublication Biology.. v2024.
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2016PAK1 translocates into nucleus in response to prolactin but not to estrogen. Biochemical and biophysical research communications. 473:206.
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2016
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2015A derivative of differentiation-inducing factor-3 inhibits PAK1 activity and breast cancer cell proliferation. International journal of cancer and clinical research. 2:1.
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2015JAK2 tyrosine kinase phosphorylates and is negatively regulated by centrosomal protein Ninein. Molecular and cellular biology. 35:111.
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2015Phosphorylation of tyrosine 285 of PAK1 facilitates $beta$PIX/GIT1 binding and adhesion turnover. The FASEB Journal. 29:943.
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2015Src tyrosyl phosphorylates cortactin in response to prolactin. Biochemical and biophysical research communications. 463:644.
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2013
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2013Tyrosyl phosphorylated PAK1 regulates breast cancer cell motility in response to prolactin through filamin A. Molecular endocrinology. 27:455.
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2011Adapter protein SH2B1$beta$ binds filamin A to regulate prolactin-dependent cytoskeletal reorganization and cell motility. Molecular endocrinology. 25:1231.
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2011PAK1-Nck regulates cyclin D1 promoter activity in response to prolactin. Molecular Endocrinology. 25:1565.
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2009Investigating the role of Aurora kinases in RAS signaling. . J. Cell Biochem.. 106:33-41.
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2009Adapter protein SH2B1$beta$ cross-links actin filaments and regulates actin cytoskeleton. Molecular endocrinology. 23:1065.
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2007
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2007Identification of the glomerular podocyte as a target for growth hormone action. Endocrinology. 148:2045.
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2007JAK2 tyrosine kinase phosphorylates PAK1 and regulates PAK1 activity and functions. Journal of Biological Chemistry. 282:30985.
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2003YXXL motifs in SH2-B$beta$ are phosphorylated by JAK2, JAK1, and platelet-derived growth factor receptor and are required for membrane ruffling. Journal of Biological Chemistry. 278:11970.
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2002Dynamics of Cytoskeletal Proteins during Fc$gamma$ Receptor-mediated Phagocytosis in MacrophagesV⃞. Molecular Biology of the Cell.
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2002Dynamics of cytoskeletal proteins during Fc$gamma$ receptor-mediated phagocytosis in macrophages. Molecular biology of the cell. 13:402.
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2002SH2-B$beta$ is a Rac-binding protein that regulates cell motility. Journal of Biological Chemistry. 277:10669.
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2000Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes. The EMBO journal. 19:199.
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2000SH2-B is required for growth hormone-induced actin reorganization. Journal of Biological Chemistry. 275:13126.
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1997Localization of five annexins in J774 macrophages and on isolated phagosomes. Journal of Cell Science. 110:1199.
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1995
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Article (Web of Science)
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2016Prolactin-induced PAK1 tyrosyl phosphorylation promotes FAK dephosphorylation, breast cancer cell motility, invasion and metastasis. BMC CELL BIOLOGY. 17.Full Text via DOI: 10.1186/s12860-016-0109-5
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2016Synergistic Activation of ER alpha by Estrogen and Prolactin in Breast Cancer Cells Requires Tyrosyl Phosphorylation of PAK1. CANCER RESEARCH. 76:2600-2611.Full Text via DOI: 10.1158/0008-5472.CAN-15-1758
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2016PAK1 translocates into nucleus in response to prolactin but not to estrogen. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 473:206-211.Full Text via DOI: 10.1016/j.bbrc.2016.03.079
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2016Synergistic Activation of ERα by Estrogen and Prolactin in Breast Cancer Cells Requires Tyrosyl Phosphorylation of PAK1. CANCER RESEARCH. 76:2600-2611.Full Text via DOI: 10.1158/0008-5472.can-15-1758
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2015Src tyrosyl phosphorylates cortactin in response to prolactin. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 463:644-649.Full Text via DOI: 10.1016/j.bbrc.2015.05.116
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2015Phosphorylation of tyrosine 285 of PAK1 facilitates beta PIX/GIT1 binding and adhesion turnover. FASEB JOURNAL. 29:943-959.Full Text via DOI: 10.1096/fj.14-259366
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2015JAK2 Tyrosine Kinase Phosphorylates and Is Negatively Regulated by Centrosomal Protein Ninein. MOLECULAR AND CELLULAR BIOLOGY. 35:111-131.Full Text via DOI: 10.1128/MCB.01138-14
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2015Tyrosyl Phosphorylated Serine-Threonine Kinase PAK1 is a Novel Regulator of Prolactin-Dependent Breast Cancer Cell Motility and Invasion. RECENT ADVANCES IN PROLACTIN RESEARCH. 846:97-137.Full Text via DOI: 10.1007/978-3-319-12114-7_5
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2015JAK2 Tyrosine Kinase Phosphorylates and Is Negatively Regulated by Centrosomal Protein Ninein. MOLECULAR AND CELLULAR BIOLOGY. 35:111-131.Full Text via DOI: 10.1128/mcb.01138-14
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2013PAK1 Regulates Breast Cancer Cell Invasion through Secretion of Matrix Metalloproteinases in Response to Prolactin and Three-Dimensional Collagen IV. MOLECULAR ENDOCRINOLOGY. 27:1048-1064.Full Text via DOI: 10.1210/me.2012-1322
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2013Tyrosyl Phosphorylated PAK1 Regulates Breast Cancer Cell Motility in Response to Prolactin through Filamin A. MOLECULAR ENDOCRINOLOGY. 27:455-465.Full Text via DOI: 10.1210/me.2012-1291
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2011PAK1-Nck Regulates Cyclin D1 Promoter Activity in Response to Prolactin. MOLECULAR ENDOCRINOLOGY. 25:1565-1578.Full Text via DOI: 10.1210/me.2011-0062
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2011Adapter Protein SH2B1 beta Binds Filamin A to Regulate Prolactin-Dependent Cytoskeletal Reorganization and Cell Motility. MOLECULAR ENDOCRINOLOGY. 25:1231-1243.Full Text via DOI: 10.1210/me.2011-0056
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2009Adapter Protein SH2B1 beta Cross-Links Actin Filaments and Regulates Actin Cytoskeleton. MOLECULAR ENDOCRINOLOGY. 23:1065-1076.Full Text via DOI: 10.1210/me.2008-0428
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2009Investigating the role of Aurora Kinases in RAS signaling. JOURNAL OF CELLULAR BIOCHEMISTRY. 106:33-41.Full Text via DOI: 10.1002/jcb.21974
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2007JAK2 tyrosine kinase phosphorylates PAK1 and regulates PAK1 activity and functions. JOURNAL OF BIOLOGICAL CHEMISTRY. 282:30985-30996.Full Text via DOI: 10.1074/jbc.M701794200
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2007Adapter protein SH2-B beta stimulates actin-based motility of Listera monocytogenes in a vasodilator-stimulated phosphoprotein (VASP)-dependent fashion. INFECTION AND IMMUNITY. 75:3581-3593.Full Text via DOI: 10.1128/IAI.00214-07
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2007Identification of the glomerular podocyte as a target for growth hormone action. ENDOCRINOLOGY. 148:2045-2055.Full Text via DOI: 10.1210/en.2006-1285
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2007Adapter Protein SH2-Bβ Stimulates Actin-Based Motility of Listeria monocytogenes in a Vasodilator-Stimulated Phosphoprotein (VASP)-Dependent Fashion. INFECTION AND IMMUNITY. 75:3581-3593.Full Text via DOI: 10.1128/iai.00214-07
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2007JAK2 Tyrosine Kinase Phosphorylates PAK1 and Regulates PAK1 Activity and Functions. JOURNAL OF BIOLOGICAL CHEMISTRY. 282:30985-30996.Full Text via DOI: 10.1074/jbc.m701794200
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2003YXXL Motifs in SH2-Bॆ Are Phosphorylated by JAK2, JAK1, and Platelet-derived Growth Factor Receptor and Are Required for Membrane Ruffling. JOURNAL OF BIOLOGICAL CHEMISTRY. 278:11970-11978.Full Text via DOI: 10.1074/jbc.m210765200
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2002Dynamics of Cytoskeletal Proteins during Fcγ Receptor-mediated Phagocytosis in Macrophages. MOLECULAR BIOLOGY OF THE CELL. 13:402-411.Full Text via DOI: 10.1091/mbc.01-05-0273
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2002SH2-Bβ Is a Rac-binding Protein That Regulates Cell Motility. JOURNAL OF BIOLOGICAL CHEMISTRY. 277:10669-10677.Full Text via DOI: 10.1074/jbc.m111138200
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2000Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes. EMBO JOURNAL. 19:199-212.Full Text via DOI: 10.1093/emboj/19.2.199
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2000SH2-B Is Required for Growth Hormone-induced Actin Reorganization. JOURNAL OF BIOLOGICAL CHEMISTRY. 275:13126-13133.Full Text via DOI: 10.1074/jbc.275.17.13126
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Book (Faculty180)
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2015Main editor "Recent Advances in Prolactin Research" book, wrote Preface. SPRINGER-VERLAG BERLIN HEIDELBERGER PLATZ 3, D-14197 BERLIN, GERMANY.
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Chapter (Faculty180)
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2020Prolactin-induced PAK1 tyrosyl phosphorylation promotes FAK dephosphorylation, breast cancer cell motility, invasion and metastasis. . Prime Archives in Molecular Biology. Ed. S. G. Zheng, Vide Leaf.
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2015Tyrosyl phosphorylated serine-threonine kinase PAK1 is a novel regulator of prolactin-dependent breast cancer cell motility and invasion. Recent Advances in Prolactin Research. Springer. 97.
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2014Prolactin Regulates Cyclin D1 Promoter Activity via Serine-threonine Kinase PAK1 and Adapter Protein Nck. "Endocrine Diseases". Concept Press Ltd.. 121-145.
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Conference Poster (Faculty180)
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Dissertation (Faculty180)
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Meeting Abstract (Web of Science)
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2014
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2013Prolactin-mediated tyrosine phosphorylation of PAK1 regulates adhesion of breast cancer cells.. MOLECULAR BIOLOGY OF THE CELL.
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2010Prolactin regulates actin cytoskeleton through JAK2 tyrosine kinase and JAK2 downstream targets. ENDOCRINE JOURNAL. S350-S350.
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2008JAK2-dependent phosphorylation of PAK1 enhances cell survival, cell motility and cell ruffling. FEBS JOURNAL. 304-304.
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Presentation (Faculty180)
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Proceedings (Faculty180)
Contact
full name
- Maria Diakonova