Holman, David H; Turner, Lorianne S; El-Zawahry, Ahmed; Elojeimy, Saeed; Liu, Xiang; Bielawski, Jacek; Szulc, Zdzislaw M; Norris, Kristi; Zeidan, Youssef H; Hannun, Yusuf A; Bielawska, Alicja; Norris, James S
description
Alterations in ceramide metabolism have been reported in prostate cancer (PCa), resulting in escape of cancer cells from ceramide-induced apoptosis. Specifically, increased expression of lysosomal acid ceramidase (AC) has been shown in some primary PCa tissues and in several PCa cell lines. To determine if this represents a novel therapeutic target, we designed and synthesized LCL204, a lysosomotropic analog of B13, a previously reported inhibitor of AC METHODS: Prostate cancer cell lines were treated with LCL204 for varying times and concentrations. Effects of treatment on cytotoxicity, sphingolipid content, and apoptotic markers were assessed.
