Chemical synthesis and immunological evaluation of entirely carbohydrate conjugate Globo H-PS A1 Article (Faculty180)

cited authors

  • Ghosh, Samir; Trabbic, Kevin R; Shi, Mengchao; Nishat, Sharmeen; Eradi, Pradheep; Kleski, Kristopher A; Andreana, Pete R

description

  • An anticancer, entirely carbohydrate conjugate, Globo H-polysaccharide A1 (Globo H-PS A1), was chemically prepared and immunologically evaluated in C57BL/6 mice. Tumor associated carbohydrate antigen Globo H hexasaccharide was synthesized in an overall 7.8% yield employing a convergent [3 + 3] strategy that revealed an anomeric aminooxy group used for conjugation to oxidized PS A1 an oxime linkage. Globo H-PS A1, formulated with adjuvants monophosphoryl lipid A and TiterMax┬« Gold. After immunization an antigen specific immune response was observed in ELISA with anti-Globo H IgG/IgM antibodies. Specificity of the corresponding antibodies was determined by FACS showing cell surface binding to Globo H-positive cancer cell lines MCF-7 and OVCAR-5. The anti-Globo H antibodies also exhibited complement-dependent cellular cytotoxicity against MCF-7 and OVCAR-5 cells.

publication date

  • 2020

published in

start page

  • 13052

end page

  • 13059

volume

  • 11