Szaniszlo, Peter; Wang, Nan; Sinha, Mala; Reece, Lisa M; Van Hook, James W; Luxon, Bruce A; Leary, James F
Most biological samples are cell mixtures. Some basic questions are still unanswered about analyzing these heterogeneous samples using gene expression microarray technology (MAT). How meaningful is a cell mixture's overall gene expression profile (GEP)? Is it necessary to purify the cells of interest before microarray analysis, and how much purity is needed? How much does the purification itself distort the GEP, and how well can the GEP of a small cell subset be recovered?