Influence of carbohydrate status on immune responses before and after endurance exercise Article (Web of Science)


  • To determine the effect of carbohydrate (CHO) status on immune responses after long-duration exercise, on two occasions, 10 men completed a glycogen-depleting bout of cycle ergometry followed by 48 h of either a high-CHO diet (HiCHO; 8.0 g CHO/kg) or a low-CHO diet (LoCHO; 0.5 g CHO/kg). After the 48 h, subjects completed a 60-min ride at 75% maximal O2 uptake (EX). Blood samples were taken predepletion, pre-EX, post-EX, and 2 and 24 h post-EX and were assayed for leukocyte number and function, glucose, glutamine, and cortisol. The glucose responses were significantly higher in the HiCHO (4.62 ± 0.26 mM) vs. the LoCHO (3.19 ± 0.15 mM) condition post-EX, and glutamine was significantly higher in the HiCHO (0.472 ± 0.036 mM) vs. the LoCHO (0.410 ± 0.025 mM) condition throughout. Cortisol levels were significantly greater in the LoCHO (587 ± 50 nM) vs. the HiCHO (515 ± 62 nM) condition throughout the trial. Lymphocyte proliferation (phytohemagglutinin) was significantly depressed after exercise. However, there was no difference between conditions, and the depression was not correlated with elevations in cortisol. Circulating numbers of leukocytes, neutrophils, lymphocytes, and lymphocyte subsets were significantly greater in the LoCHO vs. the HiCHO condition at the post-EX and 2 h post-EX time points. These data indicate that the exercise and diet manipulation altered the number of circulating leukocytes but did not affect the decrease in lymphocyte proliferation that occurred after exercise.


  • Mitchell, J. B.
  • Pizza, Francis X
  • Paquet, A.
  • Davis, B. J.
  • Forrest, M. B.
  • Braun, W. A.

publication date

  • 1998

published in

number of pages

  • 8

start page

  • 1917

end page

  • 1925


  • 84


  • 6