Infrared study of water in a poly(ethylene-co-tetrafuloroethylene) anion exchange membrane at different level of hydration (%RH 0, 25, 50, 75 and 100) was performed using a Fourier transform infrared (FTIR) microscope. FTIR spectra showed at least two different states of water present in the membrane. The presence of both the non-hydrogen bonded water molecules at the lower hydration level and the hydrogen bonded water molecules at the higher hydration level, unambiguously, are altered with changing humidity.