Silicon (Si) absorption is highly variable among different plant types; however, few studies have examined variations among different cultivars within a single species. In this study, 10 different tomato cultivars, including determinants and indeterminants as well as hybrids and heirlooms, were hydroponically grown in the presence or absence of Si to determine the absorption and distribution of the nutrients in roots, stems, petioles, and leaves. A total elemental analysis revealed that Si concentrations significantly increased with Si treatment, and that root concentrations were significantly higher than those in leaves. Although a few species showed differences in carbon, nitrogen, and calcium concentrations in roots and leaves with Si treatment, many of the macronutrients and micronutrients were unaffected. These data suggest that tomato plants absorb Si within the macronutrient range and restrict its movement from roots to shoots.