Antiproliferative Efficacy of -(3-chloro-4-fluorophenyl)-6,7-dimethoxyquinazolin-4-amine, DW-8, in Colon Cancer Cells Is Mediated by Intrinsic Apoptosis
Article (Faculty180)
Neupane, Rabin; Malla, Saloni; Abou-Dahech, Mariam S; Balaji, Swapnaa; Kumari, Shikha; Waiker, Digambar K; Moorthy, N S Hari Narayana; Trivedi, Piyush; Ashby, Cha R; Karthikeyan, Chandrabose; Tiwari, Amit K
description
A novel series of 4-anilinoquinazoline analogues, , were evaluated for anticancer efficacy in human breast cancer (BT-20) and human colorectal cancer (CRC) cell lines (HCT116, HT29, and SW620). The compound, , had the highest anticancer efficacy and selectivity in the colorectal cancer cell lines, HCT116, HT29, and SW620, with IC values of 8.50 ± 2.53 µM, 5.80 ± 0.92 µM, and 6.15 ± 0.37 µM, respectively, compared to the non-cancerous colon cell line, CRL1459, with an IC of 14.05 ± 0.37 µM. The selectivity index of was >2-fold in colon cancer cells incubated with vehicle. We further determined the mechanisms of cell death induced by in SW620 CRC cancer cells. (10 and 30 µM) induced apoptosis by (1) producing cell cycle arrest at the G2 phase; (2) activating the intrinsic apoptotic pathway, as indicated by the activation of caspase-9 and the executioner caspases-3 and 7; (3) nuclear fragmentation and (4) increasing the levels of reactive oxygen species (ROS). Overall, our results suggest that may represent a suitable lead for developing novel compounds to treat CRC.
