In Vivo CRISPR/Cas9-Based Targeted Disruption and Knockin of a Long Noncoding RNA Article (Faculty180)

cited authors

  • Cheng, Xi; Peters, Samuel T; Pruett-Miller, Shondra M; Saunders, Thomas L; Joe, Bina

description

  • The CRISPR/Cas9 system has been widely used as an efficient genome-editing tool for studying physiological functions of long noncoding RNAs (lncRNAs). In this chapter, we describe the experimental procedures for using the CRISPR/Cas9 system to genetically modify a long noncoding RNA in vivo through the targeted disruption and knockin approaches.

publication date

  • 2021

published in

start page

  • 305

end page

  • 321

volume

  • 2254